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DNA replication in short-time organ cultures of human epidermis

Inhibition by aphidicolin, and detection of DNA polymerases α, β, and γ

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Summary

Human epidermis uncontaminated by fibroblasts was isolated by a suction blister method. DNA synthesis in short-time organ cultures of isolated epidermis was strongly inhibited by aphidicolin, suggesting that DNA polymerase α is involved in DNA replication in human epidermis. On the basis of their responses to inhibitors, primer-template requirements, and chromatographic properties, DNA polymerases α, β, and γ were all identified in epidermal extracts.

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Authors and Affiliations

  1. Institute of medical Biology, University of Tromsø, N-9000, Tromsø, Norway

    H. Krokan

  2. Dept. of Dermatology, University of Tromsø, N-9000, Tromsø, Norway

    G. Volden

Authors
  1. H. Krokan
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  2. G. Volden
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Supported by the Erna and Olav Aakres Foundation for Cancer Research

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Krokan, H., Volden, G. DNA replication in short-time organ cultures of human epidermis. Arch Dermatol Res 273, 97–101 (1982). https://doi.org/10.1007/BF00509032

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  • DOI: https://doi.org/10.1007/BF00509032

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