Summary
Doses of 3 mg/kg Ro 10-9359 (in arachis oil) were daily administered to adult female Wistar rats by gastric tube for a period of 10 days. Control animals received corresponding quantities of arachis oil only. The body weight of all rats was registered daily. Samples of jejunum and ileum were processed for quantitative histochemical analysis of neutral α-glucosidase kinetics and for three-dimensional evaluation of the mucosal architecture. In addition, mucosal scrapings were prepared from these intestinal segments, and the specific sucrase activity was determined. For each animal data were pooled and analyzed by Wilcoxon (Wn) test.
Body weight and all registered parameters in the jejunum of treated animals remained unchanged as compared to the controls. In the ileum, however, we found under aromatic retinoid an increase of sucrase activity (P=0.02) and of mucosal surface per unit serosal area (P<0.05). The hydrolytic activity of neutral α-glucosidase (V max) showed a clear trend to increase at both the villus base and apex, whereas the apparent substrate affinity (K m ) remained unaltered. Our results show that, in closes of 3 mg/kg/day, aromatic retinoid induces (1) an increase in mucosal surface area, apparently due to hyperproliferation of the absorptive epithelium in ileum, which could facilitate its absorptive capacity and (2) an increase of specific sucrase activity, which could result in an enhanced carbohydrate assimilation. These findings indicate that Ro 10-9359 in addition to its effects on keratinizing epithelia exerts a distinct influence on the structure and function of the intestinal epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alpers DH, Seetharam B (1977) Pathophysiology of diseases involving intestinal brush-border proteins. N Engl J Med 296:1047–1050
Batt RM, Bush BM, Peters TJ (1979) Biochemical changes in the jejunal mucosa of dogs with naturally occurring exocrine pancreatic insufficiency. Gut 20:709–715
Bieri JG, Wu AL, Tolliver TJ (1981) Reduced intestinal absorption of Vitamin E by low dietary levels of retinoic acid in rats. J Nutr 111:458–467
Clarke RM (1970) Mucosal architecture and epithelial cell production rate in the small intestine of the albino rat. J Anat 107:519–529
Crain FD, Lotspeich FJ (1967) Biosynthesis of retinoic acid by intestinal enzymes of the rat. J Lipid Res 8:249–254
Dahlquist A (1968) Assay of intestinal disaccharidases. Anal Biochem 22:99–107
Elias PM, Williams ML (1981) Retinoids, cancer and the skin. Arch Dermatol 117:160–180
Emde C, Görge HH, Riecken EO (1981) Is ileal hyperplasia in hyperthyroid rats caused by hyperphagia? (abstract). In: Dowling RH, Riecken EO, Robinson JWL (eds) Intestinal adaptation. Proc Sec Int Conf 1981. Schattauer, Stuttgart New York
Gangl A, Pavelka M, Stöckinger L, Grabner G (1976) Fettresorption: Aktuelle Konzepte und Mikromorphologie. Z Gastroenterol 14:626–637
Gutschmidt S, Kaul W, Riecken EO (1979) A quantitative histochemical technique for the characterisation of α-glucosidase in the brush-border membrane of rat jejunum. Histochemistry 63:81–101
Gutschmidt S, Emde C, Riecken EO (1980) Quantification of α-glucosidases along the villus of the small intestine in man. Introduction of a computerized histochemical method. Histochemistry 67:85–97
Gutschmidt S (1981) In situ determinations of apparent K m and V max of brush border disaccharidases along the villi of normal human jejunal biopsy specimens. Histochemistry 71:451–462
Gutschmidt S, Emde C (1981) Early changes in brush border disaccharidase kinetics in rat jejunum following subcutaneous administration of tetraiodothyronine. A qantitative histochemical study on villi revealing normal morphology. Histochemistry 73:151–160
Gutschmidt S, Lange U, Riecken EO (1981) In situ measurments of protein contents in the brush border region along rat jejunal villi and their correlations with four enzyme activities. Histochemistry 72:467–479
Hänni R (1978) Pharmacokinetic and metabolic pathways of systemically applied retinoids. Dermatologica [Suppl I] 157:5–10
Kim HJ, Hiroi Y, Natori Y (1976) The state of lysosomes and protein turnover in rat liver. Effect of excess vitamin A. J Biochem 79:803–808
Kretchmer N, Latimer JS, Raul F, Berry K, Legum C, Sharp HL (1979) Sucrase and cellular development. In: Development of mammalian absorptive processes (Ciba Foundation Symp 70). Excerpta Medica, Amsterdam Oxford New York, pp 117–131
Kylberg H, Ong DE, Chytil F (1978) Vitamin A binding proteins during perinatal development of the intestine (abstract). Fed Proc 37:708
Lorenz-Meyer H, Köhn R, Riecken EO (1976) Vergleich verschiedener morphometrischer Methoden zur Erfassung der Schleimhautoberfläche des Rattendünndarms und deren Beziehung zur Funktion. Histochemistry 49:123–129
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193:265–275
Menge H, Robinson JWL, Riecken EO (1976) Anpassungsmöglichkeiten der Dünndarmschleimhaut an verschiedene intraluminale Milieu veränderungen. Z Gastroenterol 14:420–433
Menge H, Robinson JWL (1978) The relationship between the functional and structural alterations in the rat small intestine following proximal resection of varying extents. Res Exp Med(Berl) 173:41–53
Menge H, Riecken EO, Robinson JWL (1979) Structural and functional alterations in the mucosa of self-filling intestinal blind loops in rats. Clin Sci 56:121–131
Orfanos CE, Braun-Faclo O, Farber EM, Grupper Ch, Polano MK, Schuppli R (1981) Retinoids: Advances in basic research and therapy. Springer, Berlin Heidelberg New York
Paravicini U (1981) Pharmacokinetics and metabolism of oral aromatic retinoids. In: Orfanos CE, Braun-Falco O, Farber EM, Grupper Ch, Polano MK, Schuppli R (eds) Retinoids. Advances in basic research and therapy. Springer, Berlin Heidelberg New York, pp 13–20
Perumal AS, Lakshmanan MR, Cama HR (1968) Effects of vitamins A and K on colon lysosomes. Biochim Biophys Acta 170:399–408
Rijke RPC, Plaisier H, Hoogeveen AT, Lamerton LF, Galjaard H (1975) The effect of continuous irradiation on cell proliferation and maturation in small intestinal epithelium. Cell Tiss Kinet 8:441–453
Robinson JWL, Menge H, Schroeder P, Riecken EO, v. Melle G (1980) Structural and functional correlations in the atrophic mucosa of self-emptying blind loops of rat jejunum. Eur J Clin Invest 10:393–399
Sporn MB, Dunlop NM, Newton DL, Smith JM (1976) Prevention of chemical carcinogenesis by vitamin A on synthetic analogs (retinoids). Fed Proc 35:1332–1338
Tsambaos D, Orfanos CE (1981) Effects of oral retinoid on dermal components in human and animal skin. In: Orfanos CE, Braun-Falco O, Farber EM, Grupper Ch, Polano MK, Schuppli R (eds) Retinoids. Advances in basic research and therapy. Springer, Berlin Heidelberg New York, pp 99–108
Wang CC, Straight S, Hill DL (1976) Destabilization of mouse liver lysosomes by vitamin A compounds and analogues. Biochem Pharmacol 25:471–475
Wollaeger EE (1973) Role of the ileum in fat absorption. Mayo Clin Proc 48:836
Wu AL, Bennett Clark S Holt PR (1980) Composition of lymph chylomicrons from proximal or distal rat small intestine. Am J Clin Nutr 33:582–589
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft, grant no. Gu 184/1
Rights and permissions
About this article
Cite this article
Gutschmidt, S., Tsambaos, D. Effects of aromatic retinoid on non-keratinizing (intestinal) epithelium: Biochemical and morphological studies. Arch Dermatol Res 273, 85–90 (1982). https://doi.org/10.1007/BF00509030
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00509030