, Volume 70, Issue 1, pp 7–18 | Cite as

Ultrastructural localization of acetylcholinesterase activity in primary cultures of rat substantia nigra

  • P. Liesi
  • P. Panula
  • L. Rechardt


Ultrastructural localization of acetylcholinesterase activity was studied in primary cultures of the substantia nigra microdissected from newborn rat brains. Light microscopic observations were also made on the characteristics of dopamine neurones and acetylcholinesterase containing cells in these cultures. Ultrastructurally acetylcholinesterase activity was localized in the nuclear envelope and rough endoplasmic reticulum of neurones, which had deeply infolded, round or oval nucleus, a prominent Golgi apparatus and varying amounts of rough endoplasmic reticulum. In the neuropil acetylcholinesterase activity was seen within microtubules of neuronal processes and in the rough endoplasmic reticulum of dendrites. The enzyme activity was also demonstrated within the nuclear envelope and rough endoplasmic reticulum of probably capillary endothelial cells. Dopaminergic neurones were identified on the basis of the green catecholamine fluorescence they exhibited. Small dopaminergic neurones could be observed and there was indirect evidence that these cells did not stain for acetylcholinesterase.


Dopamine Primary Culture Substantia Nigra Dopaminergic Neurone Golgi Apparatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • P. Liesi
    • 1
  • P. Panula
    • 1
  • L. Rechardt
    • 1
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland

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