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Histochemistry

, Volume 70, Issue 1, pp 7–18 | Cite as

Ultrastructural localization of acetylcholinesterase activity in primary cultures of rat substantia nigra

  • P. Liesi
  • P. Panula
  • L. Rechardt
Article

Summary

Ultrastructural localization of acetylcholinesterase activity was studied in primary cultures of the substantia nigra microdissected from newborn rat brains. Light microscopic observations were also made on the characteristics of dopamine neurones and acetylcholinesterase containing cells in these cultures. Ultrastructurally acetylcholinesterase activity was localized in the nuclear envelope and rough endoplasmic reticulum of neurones, which had deeply infolded, round or oval nucleus, a prominent Golgi apparatus and varying amounts of rough endoplasmic reticulum. In the neuropil acetylcholinesterase activity was seen within microtubules of neuronal processes and in the rough endoplasmic reticulum of dendrites. The enzyme activity was also demonstrated within the nuclear envelope and rough endoplasmic reticulum of probably capillary endothelial cells. Dopaminergic neurones were identified on the basis of the green catecholamine fluorescence they exhibited. Small dopaminergic neurones could be observed and there was indirect evidence that these cells did not stain for acetylcholinesterase.

Keywords

Dopamine Primary Culture Substantia Nigra Dopaminergic Neurone Golgi Apparatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andén NE, Carlsson A, Dahlström A, Fuxe K, Hillarp NÅ, Larsson K (1964) Demonstration and mapping out of nigro-neostriatal dopamine neurons. Life Sci 3:523–530Google Scholar
  2. Andén NE, Dahlström A, Fuxe K, Larsson K (1965) Further evidence for the presence of nigroneostriatal dopamine neurons in the rat. Am J Anat 116:329–334Google Scholar
  3. Andén NE, Fuxe K, Hamberger B, Hökfelt T (1966) A quantitative study on the nigro-neostriatal dopamine neuron system in the rat. Acta Physiol Scand 67:306–312Google Scholar
  4. Bak IJ (1967) The ultrastructure of the substantia nigra and caudate nucleus of the mouse and the cellular localization of catecholamines. Exp Brain Res 3:40–57Google Scholar
  5. Butcher LL, Talbot K, Bilezikjian L (1975) Acetylcholinesterase neurones in dopamine-containing regions of the brain. J Neural Transm 37:127–153Google Scholar
  6. Butcher LL, Marchand R (1978) Dopamine neurons in pars compacta of the substantia nigracontain acetylcholinesterase: Histochemical correlations on the same brain section. Eur J Pharmacol 52:415–417Google Scholar
  7. Coyle JT, Axelrod J (1972) Tyrosine hydroxylase in rat brain. Developmental characteristics. J Neurochem 19:1117–1123Google Scholar
  8. Coyle JT, Jacobowitz D, Klein D, Axelrod J (1973) Dopaminergic neurons in explants of substantia nigra in culture. J Neurobiol 4:461–470Google Scholar
  9. Dahlström A, Fuxe K (1964) Evidence for the existence of monoaminecontaining neurons in the central nervous system. Acta Physiol Scand (Suppl) 62:232Google Scholar
  10. Faull RLM, Mehler WR (1978) The cells of origin of nigrofectal, nigrothalamic and nigrostriatal projections in the rat. Neuroscience 3:989–1002Google Scholar
  11. Flumerfelt BA, Lewis PR, Gwyn DG (1973) Cholinesterase activity of capillaris in the rat brain. A light and electron microscopic study. Histochem J 5:67–77Google Scholar
  12. Francois C, Percheron G, Yelnik J, Heyner S (1979) Demonstration of the existence of small local circuit neurons in the Golgi-stained primate substantia nigra. Brain Res 172:160–164Google Scholar
  13. Geffen LB, Jessell TM, Cuello AC, Iversen LL (1976) Release of dopamine from dendrites in rat substantia nigra. Nature 260:258–260Google Scholar
  14. Gulley RL, Wood RL (1971) The fine structure of the neurons in the rat substantia nigra. Tissue Cell 3:675–690Google Scholar
  15. Hajdu F, Hassler R, Bak IJ (1973) Electron microscopic study of the substantia nigra and the strio-nigral projection in the rat. Z Zellforsch 146:207–221Google Scholar
  16. Hervonen H, Rechardt L (1974) Observations on closed tissue cultures of sympathetic ganglia of chick embryos in media buffered with N-tris (hydroxymethyl)methyl-glycine or N-2-Hydroxy-Ethyl-piperazine-N-2-Ethanesulfonic Acid. Acta Physiol Scand 90:267–277Google Scholar
  17. Hornykiewicz O (1963) Die topische Lokalisation und das Verhalten von Noradrenalin und Dopamin (3-hydroxytyramin) in der Substantia nigra des normalen und Parkinson-kranken Menschen. Wien Klin Wochenschr 75:309–312Google Scholar
  18. Juraska JM, Wilson CJ, Groves PM (1977) The substantia nigra of the rat. A Golgi study. J Comp Neurol 172:585–600Google Scholar
  19. Kreutzberg GW, Kaiya H, Toth L (1979) Distribution and origin of acetylcholinesterase activity in the capillaries of the brain. Histochemistry 61:111–122Google Scholar
  20. Lehmann J, Fibiger HC (1978) Acetylcholinesterase in the substantia nigra and caudate-putamen of the rat. Properties and localization in dopaminergic neurons. J Neurochem 30:615–624Google Scholar
  21. Lewis PR, Shute CCD (1966) The distribution of cholinesterase in cholinergic neurons demonstrated with the electron microscope. J Cell Sci 1:381–390Google Scholar
  22. Lindvall O, Björklund A (1974) The glyoxylic acid fluroescence histochemical method. A detailed account of the methodology for the visualization of central catecholamine neurons. Histochemistry 39:97–127Google Scholar
  23. Loizou LA (1969) The development of monoamine-containing neurones in the brain of the albino rat. J Anat 104:588Google Scholar
  24. Loizou LA (1972) The postnatal ontogeny of monoamine-containing neurones in the central nervous system of the albino rat. Brain Res 40:395–418Google Scholar
  25. Manocha SL (1970) Histochemical distribution of acetylcholinesterase and simple esterases in the brain of squirrel monkey (Saimiri sciureus). Histochemie 21:236–248Google Scholar
  26. McCarthy KD, deVellis J (1978) Alpha-adrenergic receptor modulation of beta-adrenergic, adenosine and prostaglandin E1 increased adenosine 3′∶5′ — cyclic monophosphate levels in primary cultures of glia. J Cyclic Nucleotide Res 4:15–26Google Scholar
  27. McLennan H, York DH (1967) The action of dopamine on neurones of the caudate nucleus. J Physiol 189:393–402Google Scholar
  28. Meibach RC, Weaver LM (1979) Histochemical identification of acetylcholinesterase in dopaminergic nigrostriatal neurons. J Neural Transm 44:87–96Google Scholar
  29. Olson L, Seiger Å, Fuxe K (1972) Heterogeneity of striatal and limbic dopamine innervation: Highly fluorescent Islands in developing and adult rats. Brain Res 44:283–288Google Scholar
  30. Panula P, Rechardt L, Hervonen H (1979) Observations on the morphology and histochemistry of the rat neostriatum in tissue culture. Neuroscience 4:235–248Google Scholar
  31. Pettman B, Delaunoy JP, Courageot J, Devilliers G, Sensenbrenner M (1980) Rat brain glial cells in culture. Effect of brain extracts on the development of oligodendroglia-like cells. Dev Biol 75:278–287Google Scholar
  32. Prochiantz A, DiPorzio U, Kato A, Berger B, Glowinski J (1979) In vitro maturation of mesenchephalic dopaminergic neurons from mouse embryos is enhanced in the presence of their striatal target cells. Proc Natl Acad Sci USA 76:5387–5391Google Scholar
  33. Rinvik E, Grofová I (1970) Observations on the fine structure of the substantia nigra in the cat. Exp Brain Res 11:229–248Google Scholar
  34. Shute CCD, Lewis PR (1967) The ascending cholinergic reticular system. Neocortical, olfactory and subcortical projections. Brain 90:497–520Google Scholar
  35. Silver A (1974) The biology of cholinesterases. In: Neuberger A, Tatum EL (eds) Frontiers of biology, vol 36. North Holland American Elsevier. Amsterdam Oxford New York, pp 180–376Google Scholar
  36. Stadler H, Lloyd KG, Gadea-Ciria M, Bartholini G (1973) Enhanced striatal acetylcholine release by chlorpromazine and its reversal by apomorphine. Brain Res 55:476–480Google Scholar
  37. Tennyson VM, Mytilineou C, Barrett RE (1973) Fluorescence and electron microscopic studies of the early development of the substantia nigra and area ventralis tegmenti in the fetal rabbit. J Comp Neurol 149:233–258Google Scholar
  38. Ungerstedt U (1971) Stereotaxic mapping of the monoamine pathways in the rat brain. Acta Physiol Scand Suppl 367Google Scholar
  39. Wilson CJ, Groves PM, Fifkova E (1977) Monoaminergic synapses, including dendro-dendritic synapses in the rat substantia nigra. Exp Brain Res 30:161–174Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • P. Liesi
    • 1
  • P. Panula
    • 1
  • L. Rechardt
    • 1
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland

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