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Occurrence and evolutionary significance of two sulfate assimilation pathways in the rhodospirillaceae

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Abstract

The ability to use adenosine 5′-phosphosulfate (APS) or 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as the substrate for the initial reductive step in sulfate assimilation has been tested in most of the known Rhodospirillaceae species and in some chemotrophic bacteria. Improved and optimized methods for the synthesis and purification of the sulfonucleotides APS and PAPS are described. The production of acid volatile radioactivity from 35S-APS and 35S-PAPS was measured under various conditions in the presence and absence of non-labeled sulfate. Specific differences in the ability to reduce APS or PAPS were observed among the Rhodospirillaceae species and also the chemotrophic bacteria. APS was found to be the substrate of the thiolsulfotransferase in Rps. acidophila, Rps. globiformis, Rm. vannielii, Rc. purpureus, R. tenue, Rps. gelatinosa, in Alcaligenes eutrophus and Pseudomonas aeruginosa. PAPS was the substrate in Rps. capsulata, Rps. sphaeroides, Rps. sulfidophila, Rps. palustris, Rps. viridis, R. rubrum, R. fulvum, in Paracoccus denitrificans and in several Enterobacteriaceae. The presence of different enzymatic systems for sulfate reduction in the Rhodospirillaceae family is compared with their taxonomical grouping and their possible phylogenetic relatedness.

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Abbreviations

APS:

adenosine 5′-phosphosulfate

PAPS:

3′-phosphate adenosine 5′-phosphosulfate

DTE:

dithioerythrol

Rc.:

Rhodocyclus

R.:

Rhodospirillum

Rm.:

Rhodomicrobium

Rps.:

Rhodopseudomonas

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  1. Institut für Mikrobiologie, Meckenheimer Allee 168, D-5300, Bonn, Federal Republic of Germany

    Johannes F. Imhoff

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  1. Johannes F. Imhoff
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Imhoff, J.F. Occurrence and evolutionary significance of two sulfate assimilation pathways in the rhodospirillaceae. Arch. Microbiol. 132, 197–203 (1982). https://doi.org/10.1007/BF00508731

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  • DOI: https://doi.org/10.1007/BF00508731

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