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Lectin cytochemistry of cell types in human and canine pituitary

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Summary

Labeled lectins specific for different sugars were employed to identify different cell types in pituitaries from six human autopsies and seven dogs. To determine the lectins bound by each cell type, fixed-paraffin embedded sections serial to those stained with lectins were immunostained for specific hormones and the serial pairs were examined in a comparison microscope. In human pituitaries corticotrophs stained selectively with lectins having affinity for α-l-fucose and the core region of complex type N-glycosyl-proteins. Some corticotrophs also stained for the presence of terminal β-galactose. Thyrotrophs stained selectively with a periodate oxidation-borohydride reduction-concanavalin A sequence. Some mammotrophs evidenced content of glycoconjugate with terminal β-galactose. Dendritic cells stained selectively for abundant glycogen with the periodate-reduction-concanavalin A sequence and a lectin from Griffonia simplicifolia. Adenohypophyseal cells of dog pituitary differed in showing absence of terminal β-galactose in corticotrophs, presence of terminal β-galactose in thyrotrophs, presence of glycoconjugate with N-glycosidically bound oligosaccharide in thyrotrophs and gonadotrophs and presence of terminal β-galactose with a different lectin affinity in mammotrophs. The main contributions of lectin histochemistry applied to the pituitary include providing an additional histologic method for identification of some cell types, and localizing glycosylated prohormone or other biochemically unrecognized non-hormone glycoconjugates whose function in pituitary cells remains to be explained.

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Author notes

  1. F. Nakagawa

    Present address: Department of Neurosurgery, Shinshu University School of Medicine, 390, Matsumoto, Japan

Authors and Affiliations

  1. Department of Pathology, Medical University of South Carolina, 171 Ashley Avenue, 29425, Charleston, South Carolina, USA

    F. Nakagawa, B. A. Schulte, M. A. Sens, N. Kochibe & S. S. Spicer

  2. Department of Biology, Faculty of Education, Gunma University, 1375 Aramaki, 371, Maebashi, Gunma, Japan

    F. Nakagawa, B. A. Schulte, M. A. Sens, N. Kochibe & S. S. Spicer

Authors
  1. F. Nakagawa
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  2. B. A. Schulte
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  3. M. A. Sens
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  4. N. Kochibe
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  5. S. S. Spicer
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Additional information

This research was supported by NIH Grants AM-10956 and HL-29775 and United Health and Medical Research Foundation of South Carolina, Inc. Grant #79

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Nakagawa, F., Schulte, B.A., Sens, M.A. et al. Lectin cytochemistry of cell types in human and canine pituitary. Histochemistry 85, 57–66 (1986). https://doi.org/10.1007/BF00508654

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