Summary
Using cytophotometric procedures, we measured the nuclear and nucleolar protein content of successive zones of growth and differentiation in consecutive (1–7 mm) root segments obtained from eight species of the Angiospermae after staining the preparations with Feulgen-Naphthol Yellow S (F-NYS). In meristematic cells the nuclear and nucleolar protein content was found to double during the cell cycle. In species in which differentiation occurs at the same time as nuclear DNA endoreplication, i.e. Vicia faba subsp. minor, V. faba subsp. major, Pisum sativum, Hordeum vulgare and Amaryllis belladonna, the pool of nuclear proteins observed during the G2 phase of the cell cycle was seen in the differentiated zone in nuclei containing 8C DNA. Species in which differentiation is not accompanied by the process of nuclear DNA endoreplication, i.e. Levisticum officinale, Tulipa kaufmanniana and Haemanthus katharinae, exhibited the highest nuclear proteins content during the G2 phase of the cell cycle; comparably high values were not found in the differentiated zone. A decrease in nucleolar protein content was observed during the process of differentiation, this tendency being more evident in the studied species that do not exhibit endoreplication.
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References
Armstrong SW, Davidson D (1981) Differences in protein content of sister nuclei: evidence from binucleate and mononucleate cells. Can J Bot 80:371–378
Auer G, Ono J, Caspersson TO (1983) Determination of the fraction of Go cells in cytologic tamples by means of simultaneous DNA and nuclear protein analyses. Anal Quant Cytol 5:1–4
Barlow PW (1978) Endopolyploidy: towards an understanding of its biological significance. Acta Biotheoret 27:1–18
Barlow PW (1982) Root development. In: Smith H, Grieson D (eds) The molecular biology of plant development, Blackwell Scientific Publications, Oxford, pp 185–209
Chang PL, Georgiadis N, Joubert GJ, Davidson RG (1983) Gene dosage effect on human diploid and tetraploid fibroblasts. Exp Cell Res 145:277–284
Cohn NS, Mitchell JP, van den Broek K (1983) Nuclear and nucleolar protein during the cell cycle in differentiating Pisum sativum vascular tissue. Histochemistry 79:353–364
Frederiks WM, Slob A, Schröder M (1980a) Application of the Barr and Stroud GN5 densitometer for description of the chromatin structure. Microsc Acta 83:317–321
Frederiks WM, Slob A, Schröder M (1980b) Histochemical determination of histone and non-histone protein content in rat liver nuclei. Histochemistry 68:49–53
Gaub J (1976) Feulgen-Naphthol Yellow S cytophotometry liver cells. The effect formaldehyde induced shrinkage on nuclear Naphthol Yellow S binding. Histochemistry 49:293–301
Gaub J (1981) Quantification of nuclear non-histone proteins by Feulgen-Naphthol Yellow S cytophotometry. Histochem J 13:717–722
Jeble N, Kiefer G (1979) Incorporation of uridine into polyploid and binuclear cells of mouse liver. Eur J Cell Biol 19:89–91
Kononowicz AK (1978) RNA synthesis and changes in template — DNA activity during the growth and differentiation of parenchyma cells of the primary cortex of root of Zea mays and Tulipa kaufmanniana. Folia Histochem Cytochem 16:123–138
Kononowicz AK, Kuran H, Olszewska MJ (1976) Cytochemical and ultrastructural studies of changes in the nucleus and nucleolus during cell differentiation in the root cortex of Haemanthus katharinae. Folia Histochem Cytochem 14:151–164
Marciniak K, Bilecka A (1985) Changes in nuclear, nucleolar and cytoplasmic RNA content during growth and differentiation of root parenchyma cells in plant species with different dynamics of DNA endoreplication. Folia Histochem Cytobiol 23:231–246
Mitchell JP, van der Ploeg M, van Duijn P (1981) Combined staining procedures for cytophotometry of protein and DNA Feulgen-Naphthol Yellow S and dinitrofluorobenzene-Feulgen. Histochemistry 73:211–223
Nagl W (1972) Molecular and structural aspects of the endomitotic chromosome cycle in angiosperms. Chromosomes Today 3:17–23
Nagl W (1973) The mitotic and endomitotic nuclear cycle in Allium carinatum. IV 3H uridine incorporation. Chromosoma 44:203–212
Nagl W (1979) Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. Plant Syst Evol (Suppl) 2:247–260
Olszewska MJ (1976) Autoradiographic and ultrastructural study of Cucurbita pepo root cells during their growth and differentiation. Histochemistry 49:157–175
Olszewska MJ, Kononowicz AK (1979) Activities of DNA polymerases and RNA polymerases detected in situ in growing and differentiating cells of root cortex. Histochemistry 59:311–323
Olszewska MJ, Osiecka R (1982) The relationship between 2C DNA content, life cycle type, systematic position, and the level of DNA endoreplication in nuclei of parenchyma cells during growth and differentiation of roots in some monocotyledonous species. Biochem Physiol Pflanzen 177:319–336
Olszewska MJ, Osiecka R (1983) The relationship between 2C DNA content, life cycle type, systematic position and the dynamics of DNA endoreplication in parenchyma nuclei during growth and differentiation of roots in some dicotyledonous herbaceous species. Biochem Physiol Pflanzen 178:581–599
Olszewska MJ, Bilecka A, Kuran H, Marciniak K (1982) Cell cycle control in synchronously dividing antheridial filaments of Chara vulgaris L. as revealed by cycloheximide pulse treatment. Folia Histochem Cytochem 20:3–24
Olszewska MJ, Marciniak K, Kuran H, Damsz B, Osiecka R, Przeździecka A (1983) Changes in the content of RNA, protein and dry mass in nuclei, nucleoli and cytoplasm, and the rate of cell growth during cell cycle in root meristem of Vicia faba L. subsp. minor and subsp. major. Microse Acta 87:139–158
Olszewska MJ, Damsz B, Kuran H, Marciniak K (1984a) Enhanced rRNA transport from nucleoli into cytoplasm at G1/S, S/G2, and G2/M transitory points of the cell cycle in root meristem of Helianthus annuus L. Eur J Cell Biol 33:1–6
Olszewska MJ, Marciniak K, Kuran H, Damsz B, Bilecka A (1984b) Cytochemical study of cell cycle parameters in two cultivars of Helianthus annuus L. Dry mass increase as a possible factor regulating cell cycle duration. Capyologia 37:177–195
Tas J, James J (1981) Quantitative cytochemistry of nuclear and cytoplasmic proteins using the Naphthol Yellow S and dinitrofluorobenzene staining methods. Histochem J 13:711–716
Wagenaar EB (1983) Inhibition of protein synthesis during G2 induces irregular metaphase and anaphase in mitosis of root cells of Allium sativum L. Cell Biol Int Rep 7:827–833
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This work was supported by the Polish Academy of Sciences as a part of project no 09.7.1.4.5
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Marciniak, K., Bilecka, A. Changes in nuclear and nucleolar protein content during the growth and differentiation of root parenchyma cells in plant species with different DNA-endoreplication dynamics. Histochemistry 85, 51–56 (1986). https://doi.org/10.1007/BF00508653
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DOI: https://doi.org/10.1007/BF00508653