Summary
The skin explant model was used to determine the effect of antibody reactions against membrane associated antigens on normal human keratinocytes. Addition of specific allo-antibodies against HLA class I antigens induced characteristic changes in the cells on the outermost region of the explant-outgrowth.
A desorganization of the filopodia of these cells occurred and the edges of the cellular border were lifted from the substratum. These signs of detachment were also found when pemphigus serum was added. In both experimental conditions the detachment of the cells was complement independent. After removing the antiserum a recovery took place, but the cells once lifted from the substratum remained recognizable as a ridge of cells.
No changes were observed when the explants were incubated with antibodies against HLA class II antigens. Incubation with specific antibodies against HLA class I antigens not present on the explant had also no effect. We propose that antibody reactions against various membrane associated antigens can induce within a few hours characteristic changes of the cellular margins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartlett PF, Edidin M (1978) Effect of the H-2 gene complex rates of fibroblasts intercellular adhesion. J Cell Biol 77:377–388
Claas FHJ, Ponec M, Kardaun SH, Vermeer BJ, Van Rood JJ (1984) A new sensitive assay for the detection of HLA-antigens on human epithelial cells. Tissue Antigens 24:121–125
Czernielewski J, Dermachez M, Prunieras M (1984) Human langerhans cells in epidermal cell culture, in vitro skin explants and skin grafts onto “nude” mice. Arch Dermatol Res 276:288–292
Dabbelsteen E, Buschard K, Itiroh Hakomori S, Young WW (1984) Pattern of distribution of bloodgroups antigens on human epidermal cells during maturation. J Invest Dermatol 82:13–17
Farb RM, Dijkes R, Lazarus GS (1978) Anti-epidermal cellsurface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase. Proc Natl Acad Sci USA 75:459–463
Hashimoto K, Shafram KM, Webber PS, Lazarus GS, Singer KH (1983) Anti-cell surface pemphigus autoantibody stimulates plasminogen activator activity of human epidermal cells. J Exp Med 157:257–272
Hennings H, Holbrook KA (1983) Calcium regulation of cell-cell contact and differentiation of epidermal cells in culture. Exp Cell Res 143:127–142
Hietanen J, Salo OP, Kariniemi AJ (1983) Pemphigus serum-induced loss of microvilli from human epidermal cells. Acta Derm Venerol 63:115–121
Kawama S, Janson M, Jordon RE (1984) Complement fixation by pemphigus antibody I in vitro fixation to organ and tissue culture skin. J Invest Dermatol 82:506–510
Kissmeyer-Nielsen F, Olsen S, Petersen VP, Fjeldborg O (1966) Hyperacute rejection of kidney allografts associated with preexisting humoral antibodies against donor cells. Lancet 1:662–665
Koerten HK, Ploem JS, Daems WTh (1980) Ingestion of latex beads by filopodia of adherent mouse peritoneal macrophages. Exp Cell Res 128:470–475
Morhenn VB, Benike CJ, Cox AJ, Charron DJ, Engleman EG (1982) Cultured human epidermal cells do not synthesize HLA-Dr. J Invest Dermatol 78:32–37
Moroika S, Naito K, Ogawa H (1981) The pathogenic role of pemphigus antibodies and proteinase in epidermal acantholysis. J Invest Dermatol 76:337–341
Singer KH, Hashimoto K, Stuhlmiller GM, Lazarus GS (1982) Antibody-induced release of cellular proteinases: loss of adhesion of human melanoma cells after binding of anti-melanoma antibody. J Immunol 128:1321–1326
Vermeer BJ, Kardaun SH, Koerten HK, Claas FHJ, Ploem JS (1985) Model for detection of membrane associated antigens on epithelial cells. Br J Dermatol 113, S. 28:118–123
Vollmers HP, Birchmeier W (1983) Monoclonal antibodies that prevent adhesion of B 16 melanoma cells and reduce metastases in mice: cross-reaction with human tumor cells. Proc Natl Acad Sci USA 80:6863–6867
Weiss L (1965) Studies on cellular adhesion in tissue culture, VIII some effects of antisera on cell detachment. Exp Cell Res 37:540–551
Woo TY, Hoghan VA, Patel H, Anhalt GJ, Labib RS, Voorhees JJ, Diaz LA (1983) Specificity and inhibition of the epidermal detachment induced by pemphigus IgG in vitro. J Invest Dermatol 81:1155–1215
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vermeer, B.J., Kardaun, S.H., Wijsman, M.C. et al. Morphological studies on cellular detachment induced by antibody reactions directed against membrane associated antigens. Histochemistry 85, 35–40 (1986). https://doi.org/10.1007/BF00508651
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00508651