Summary
The investigations were carried out on 24 mongrel dogs which were anaesthetized with chloralose or sodium pentobarbital. Ouabain was added to the coronary blood (50, 100 and 200 ng/ml coronary blood or 0.7, 1.4 and 2.8×10−7 M) over periods of 30 or 60 min by intracoronary infusion of the glycoside.
Under chloralose anaesthesia, ouabain at concentrations of 100 and 200 ng/ml coronary blood augmented left ventricular dp/dt significantly. No significant changes were observed in coronary resting flow and flow per beat at the same time. Likewise, the maximum reactive hyperaemic blood flow remained constant, indicating the absence of any changes in the tone of the large extramural arteries.
Under sodium pentobarbital anaesthesia small decreases in heart rate and increases in left ventricular dp/dt were seen only at the highest ouabain concentration (200 ng/ml). All flow parameters remained unchanged.
These experiments provide evidence that concentrations of ouabain which 100- and 200-fold exceed therapeutic maintenance levels and 10- and 20-fold exceed the concentrations that produce constriction of helically cut pig and rabbit coronary arteries in vitro, do not diminish the coronary blood supply of the anaesthetized dog in vivo
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bünger, R., Haddy, F. J., Querengässer, A., Gerlach, E.: Studies on potassium induced coronary dilation in the isolated guinea pig heart. Pflügers Arch. 363, 27–31 (1976)
Campbell, R. C.: Statistische Methoden für Biologie und Medizin. Stuttgart: G. Thieme 1971
DeMots, H., McAnulty, J., Porter, G., Rahimtoola, S.: Effects of rapid and slow infusion of ouabain on systemic and coronary vascular resistance in patients without heart failure. Circulation 51/52, Sup. II, 298 (1975)
DeMots, H., Rahimtoola, S. H., Kremkau, E. L., Bennett, W., Mahler, D.: Effects of ouabain on myocardial oxygen supply and demand in patients with chronic coronary artery disease. J. Clin. Invest. 58, 312–319 (1976)
Eberlein, H. J.: Einfluß verschiedener Anaesthetica auf Koronarwiderstand und Reaktionsweise des Koronarsystems beim Hund. Anaesthesist 13, 381–384 (1964)
Fleckenstein, A., Fleckenstein-Grün, G.: Further studies on the neutralisation of glycoside-induced contractures of coronary smooth muscle by Ca-antagonistic compounds. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, R38 (1975)
Grün, G., Weder, U.: Augmentation of coronary smooth muscle tone and sensitization to Ca by cardiac glycosides. Naunyn-Schmiedeberg's Arch. Pharmacol. 282, R28 (1974)
Nakayama, K., Byon, Y. K., Fleckenstein, A.: Ca-synergistic glycoside effects on myocardial contractility and coronart vascular tone-dose-response-curves on isolated papillary muscles and coronary strips. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, R38 (1975)
Page, R. G., Foltz, E. L., Sheldon, W. F., Wendel, H.: Effect of ouabain on the coronary circulation and other circulatory functions in intact anesthetized dogs. J. Pharmacol. Exp. Ther. 101, 112–118 (1951)
Pitt, B.: Physiology and pathophysiology of the coronary circulation and the role of nitroglycerin. In: Organic Nitrates. Ed.: Philip Needleman. Berlin-Heidelberg-New York: Springer 1975
Pitt, B., Gregg, D. E.: Coronary hemodynamic effects of increasing ventricular rate in the unanesthetized dog. Circ. Res. 22, 753–761 (1968)
Raberger, G., Schütz, W., Zimpfer, M.: Blood flow in intact and constricted coronary arteries under the influence of ouabain. Naunyn-Schmiedeberg's Arch. Pharmacol. 295, 51–54 (1976)
Treat, E., Ulano, H. B., Jacobson, E. D.: Effects of intra-arterial ouabain on mesenteric and carotid hemodynamics. J. Pharmacol. Exp. Ther. 179, 144–148 (1971)
Van Citters, R. L., Franklin, D. L., Rushmer, R. F.: Left ventricular dynamics in dogs during anesthesia with alpha-chloralose and sodium pentobarbital. Am. J. Cardiol. 13, 349–354 (1964)
Vatner, St. F., Higgins, Ch. B., Franklin, D., Braunwald, E.: Effects of a digitalis glycoside on coronary and systemic dynamics in conscious dogs. Circ. Res. 28, 470–479 (1971)
Wolff, G., Grädel, E., Niederer, W.: Changes of coronary arteriolar tone, mean coronary flow and aortic pressure under halothane and ether anesthesia in the dog. Br. J. Anaesth. 40, 810–817 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zimpeer, M., Schütz, W. & Raberger, G. Haemodynamic and coronary actions of ouabain during coronary infusion. Naunyn-Schmiedeberg's Arch. Pharmacol. 299, 61–64 (1977). https://doi.org/10.1007/BF00508638
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00508638