Decreased prostaglandin synthetase activity during kidney regeneration after folic acid or 2,4,5-triamino-6-styrylpyrimidine application

  • R. M. Ingerowski
  • F. Haux
  • F. v. Bruchhausen
Article

Summary

Several pteridines (folic acid, xanthopterine and 2-amino-4-hydroxypteridine) as well as aminostyrylpyrimidine derivatives (2,4,5-triamino-6-styrylpyrimidine) cause kidney hypertrophy in experimental animals after a single parenteral application. The effect of several of these compounds on the in vivo and in vitro prostaglandin synthesis by rat kidney medulla was tested on the assumption that prostaglandins, via cAMP, might exert a growth limiting effect on the normal kidney. Of the renohypertrophic substances tested, only 2,4,5-triamino-6-styrylpyrimidine inhibited in vitro a highly active preparation of prostaglandin synthetase from rat kidney medulla, whereas folic acid, xanthopterine and 2-amino-4-hydroxypteridine were inactive. 2-Styrylpyridine also strongly inhibited prostaglandin synthetase but did not cause kidney hypertrophy. When the substances were first applied as a single dose parenterally, both 2,4,5-triamino-6-styrylpyrimidine and folic acid led to a large reduction of the prostaglandin synthesizing capacity of the rat kidney medulla as long as 14 days after drug application. 1-Styrylpyridine was inactive in this assay. The ability of the substances investigated to inhibit prostaglandin synthesis in vitro does not correlate with their renohypertrophic properties. The long-lasting reduction of the capacity to produce prostaglandins in vivo may be due to the increased growth rate seen after systemic treatment.

Key words

Prostaglandin synthesis Kidney hypertrophy Pteridines Renal medulla 2,4,5-triamino-6-styrylpyrimidine 

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • R. M. Ingerowski
    • 1
  • F. Haux
    • 1
  • F. v. Bruchhausen
    • 1
  1. 1.Pharmakologisches Institut der Freien Universität BerlinBerlin 33

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