Summary
The electron-microscopical autoradiographical resolution of a gold latensification-elon ascorbic acid (GEA) developer for IIford L4 emulsion was determined experimentally, using radioactive line sources of tritiated albumin (Heijnen and Geuze, 1977). For sections with a thickness of 62 nm (SD±11), which were covered with a carbon layer about 5 nm thick and a slightly overlapping monolayer of L4 silver bromide crystals, the measured half-distance (HD) of resolution was 115 nm. This improvement in resolution, the high efficiency of the GEA developer for L4 emulsion (Wisse and Tates, 1968), and the excellent visibility of the cellular structures under the small silver grains, mean that the L4-GEA combination deserves preverence as a method for quantitative electron-microscopical autoradiography.
This is a preview of subscription content, log in via an institution to check access.
References
Bachmann, L., Salpeter, M.M.: Autoradiography with the electron microscope. Lab. Invest. 14, 1041–1053 (1965)
Ginsel, L.A., Onderwater, J.J.M., Daems, W.Th.: Transport of radiolabelled glycoprotein to cell surface and lysosome-like bodies of absorptive cells in cultured small-intestinal tissue from normal subjects and patients with a lysosomal storage disease. Virchows Arch. B Cell Path. 30, 245–273 (1979)
Heijnen, H., Geuze, H.: Phenidone-ascorbic acid development in electronmicroscopic autoradiography. Histochemistry 54, 39–50 (1977)
Kopriwa B.M.: A comparison of various procedures for fine grain development in electron microscopic radioautography. Histochemistry 44, 201–224 (1975)
Rogers, A.W.: Techniques of autoradiography. Amsterdam: Elsevier (1973)
Salpeter, M.M., Bachmann, L.: Autoradiography with the electron microscope. J. Cell Biol. 22, 469–477 (1964)
Salpeter, M.M., Bachmann, L., Salpeter, E.E.: Resolution in electron microscope radioautography. J. Cell Biol. 41, 1–21 (1969)
Salpeter, M.M., Szabo, M.: Sensitivity in electron microscope autoradiography. I. The effect of radiation dose. J. Histochem. Cytochem. 20, 425–434 (1972)
Vrensen, G.F.J.M.: Some new aspects of efficiency of electron microscopic autoradiography with tritium. J. Histochem. Cytochem. 18, 278–290 (1970a)
Vrensen, G.F.J.M.: Further observations concerning the involvement of rough endoplasmic reticulum and ribosomes in early stages of glycogen repletion in rat liver. J. Microscopie 9, 517–534, (1970b)
Wisse, E., Tates, A.D.: A gold latensification-elon ascorbic acid developer for IIford L4 emulsion. In: Electronmicroscopy. Fourth European Region. Conf. on Electron Microscopy, Rome, 1968
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ginsel, L.A., Onderwater, J.J.M. & Daems, W.T. Resolution of a gold latensification-elon ascorbic acid developer for IIford L4 emulsion. Histochemistry 61, 343–346 (1979). https://doi.org/10.1007/BF00508456
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00508456