Summary
Nerve fibers reacting with antisera demonstrating gut-type glucagon were numerous in certain areas of hypothalamus and thalamus but absent from neocortex and hippocampus. They did not react with glucagon antisera specific for pancreatic type glucagon. Immunoreactive cell bodies were not observed.
References
Coons, D.H., Leduc, E.H., Conolly, J.M.: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. Exp. Med. 102, 49–60 (1955)
Dockray, G.J., Gregory, R.A., Hutchinson, J.B., Harris, J.I., Runswick, M.J.: Isolation, structure and biological activity of two cholecystokinin octapeptides from sheep brain. Nature 274, 711–713 (1978)
Elde, R., Hökfelt, T., Johansson, O., Terenius, L.: Immunohistochemical studies using antibodies to leucine-enkephalin: Initial observations on the nervous system of the rat. Neuroscience 1, 349–351 (1976)
Hökfelt, T., Efendié, S., hellerström, C., Johansson, O., Luft, R., Arimura, A.: Cellular localization of somatostatin in endocrine-like cells and neurons of the rat with special reference to the A1 cells of the pancreatic islets and to the hypothalamus. Acta Endocrinol. (Kbh) Suppl. 200, 5–40 (1975)
Hökfelt, T., Elde, R., Fuxe, K., Johansson, O., Ljungdahl, Å., Goldstein, M., Luft, R., Efendić, S., Nilsson, G. Terenius, L., Ganten, D., Jeffcoate, S.L., Rehfeld, J., Said, S., Perez de la Mora, M., Possani, L., Tapia, R., Teran, L., Palacios, R.: Aminergic and peptidergic pathways in the nervous system with special reference to the hypothalamus. In: Hypothalamus (eds.) S., Reichlin, R.J. Baldessarini, J.B. Martin, pp. 69–135. New York: Raven Press 1978
Holst, J.J., Aasted, B.: Production and evaluation of glucagon antibodies for radioimmunoassay. Acta Endocrinol (Kbh) 77, 715–725 (1974)
König, J.F.R., Klippel, R.A.: The rat brain. A stereotaxic atlas of the forebrain and lower parts of the brain stem. Baltimore: Williams and Wilkins 1963
Larsson, L.-I., Holst, J., Håkanson, R., Sundler, F.: Distribution and properties of glucagon immunoreactivity in the digestive tract of various mammals: An immunohistochemical and immunochemical study. Histochemistry 44, 281–290 (1975)
Ljungdahl, Å, Hökfelt, T., Nilsson, G.: Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3, 861–943 (1978)
Lorén, I., Alumets, J., Håkanson, R., Sundler, F.: Distribution of gastrin and CCK-like peptides in rat brain. Histochemistry 59, 249–257 (1979a)
Lorén, I., Emson, P., Fahrenkrug, J., Björklund, A., Alumets, J., Håkanson, R., Sundler, F.: Distribution of vasoactive intestinal polypeptide (VIP) in the rat and mouse brain. Neuroscience in press (1979b)
Pearse, A.G.E.: Peptides in brain and intestine. Nature 262, 92–94 (1976)
Rehfeld, J.F.: Immunochemical studies of cholecystokinin. II. Distribution and molecular heterogeneity in the central nervous system and small intestine of man and hog. J. Biol. Chem. 283, 4022–4030 (1978)
Schultzberg, M., Dreyfus, C.F., Gershon, M.D., Hökfelt, T., Elde, R.P., Nilsson, G., Said, S., Goldstein, M.: VIP-, enkephalin-, substance P- and somatostatin-like immunoreactivity in neurons intrinsic to the intestine: immunohistochemical evidence from organotypic tissue cultures. Brain Res. 155, 239–248 (1978)
Simantov, R., Kuhar, M.J., Pasternak, G.W., Snyder, S.H.: The regional distribution of a morphinelike factor enkephalin in monkey brain. Brain Res. 106, 189–197 (1976)
Sundler, F., Alumets, J., Håkanson, R.: Peptides in the gut with a dual distribution in nerves and endocrine cells. In: Gut Hormones. S.R. Bloom (ed.), pp. 406–413. Edinburgh: Churchill-Livingstone 1978
Tager, H.S.: Coupling of peptides to albumin with difluorodinitrobenzene. Anal. Biochem. 71, 367–375 (1976)
Author information
Authors and Affiliations
Additional information
Grant support from the Swedish Medical Research Council (04X-4499)
Rights and permissions
About this article
Cite this article
Lorén, I., Alumets, J., Håkanson, R. et al. Gut-type glucagon immunoreactivity in nerves of the rat brain. Histochemistry 61, 335–341 (1979). https://doi.org/10.1007/BF00508455
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00508455