, Volume 61, Issue 3, pp 335–341 | Cite as

Gut-type glucagon immunoreactivity in nerves of the rat brain

  • I. Lorén
  • J. Alumets
  • R. Håkanson
  • F. Sundler
  • J. Thorell
Short Communications


Nerve fibers reacting with antisera demonstrating gut-type glucagon were numerous in certain areas of hypothalamus and thalamus but absent from neocortex and hippocampus. They did not react with glucagon antisera specific for pancreatic type glucagon. Immunoreactive cell bodies were not observed.


Public Health Glucagon Nerve Fiber Cell Body Immunoreactive Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Coons, D.H., Leduc, E.H., Conolly, J.M.: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. Exp. Med. 102, 49–60 (1955)Google Scholar
  2. Dockray, G.J., Gregory, R.A., Hutchinson, J.B., Harris, J.I., Runswick, M.J.: Isolation, structure and biological activity of two cholecystokinin octapeptides from sheep brain. Nature 274, 711–713 (1978)Google Scholar
  3. Elde, R., Hökfelt, T., Johansson, O., Terenius, L.: Immunohistochemical studies using antibodies to leucine-enkephalin: Initial observations on the nervous system of the rat. Neuroscience 1, 349–351 (1976)Google Scholar
  4. Hökfelt, T., Efendié, S., hellerström, C., Johansson, O., Luft, R., Arimura, A.: Cellular localization of somatostatin in endocrine-like cells and neurons of the rat with special reference to the A1 cells of the pancreatic islets and to the hypothalamus. Acta Endocrinol. (Kbh) Suppl. 200, 5–40 (1975)Google Scholar
  5. Hökfelt, T., Elde, R., Fuxe, K., Johansson, O., Ljungdahl, Å., Goldstein, M., Luft, R., Efendić, S., Nilsson, G. Terenius, L., Ganten, D., Jeffcoate, S.L., Rehfeld, J., Said, S., Perez de la Mora, M., Possani, L., Tapia, R., Teran, L., Palacios, R.: Aminergic and peptidergic pathways in the nervous system with special reference to the hypothalamus. In: Hypothalamus (eds.) S., Reichlin, R.J. Baldessarini, J.B. Martin, pp. 69–135. New York: Raven Press 1978Google Scholar
  6. Holst, J.J., Aasted, B.: Production and evaluation of glucagon antibodies for radioimmunoassay. Acta Endocrinol (Kbh) 77, 715–725 (1974)Google Scholar
  7. König, J.F.R., Klippel, R.A.: The rat brain. A stereotaxic atlas of the forebrain and lower parts of the brain stem. Baltimore: Williams and Wilkins 1963Google Scholar
  8. Larsson, L.-I., Holst, J., Håkanson, R., Sundler, F.: Distribution and properties of glucagon immunoreactivity in the digestive tract of various mammals: An immunohistochemical and immunochemical study. Histochemistry 44, 281–290 (1975)Google Scholar
  9. Ljungdahl, Å, Hökfelt, T., Nilsson, G.: Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3, 861–943 (1978)Google Scholar
  10. Lorén, I., Alumets, J., Håkanson, R., Sundler, F.: Distribution of gastrin and CCK-like peptides in rat brain. Histochemistry 59, 249–257 (1979a)Google Scholar
  11. Lorén, I., Emson, P., Fahrenkrug, J., Björklund, A., Alumets, J., Håkanson, R., Sundler, F.: Distribution of vasoactive intestinal polypeptide (VIP) in the rat and mouse brain. Neuroscience in press (1979b)Google Scholar
  12. Pearse, A.G.E.: Peptides in brain and intestine. Nature 262, 92–94 (1976)Google Scholar
  13. Rehfeld, J.F.: Immunochemical studies of cholecystokinin. II. Distribution and molecular heterogeneity in the central nervous system and small intestine of man and hog. J. Biol. Chem. 283, 4022–4030 (1978)Google Scholar
  14. Schultzberg, M., Dreyfus, C.F., Gershon, M.D., Hökfelt, T., Elde, R.P., Nilsson, G., Said, S., Goldstein, M.: VIP-, enkephalin-, substance P- and somatostatin-like immunoreactivity in neurons intrinsic to the intestine: immunohistochemical evidence from organotypic tissue cultures. Brain Res. 155, 239–248 (1978)Google Scholar
  15. Simantov, R., Kuhar, M.J., Pasternak, G.W., Snyder, S.H.: The regional distribution of a morphinelike factor enkephalin in monkey brain. Brain Res. 106, 189–197 (1976)Google Scholar
  16. Sundler, F., Alumets, J., Håkanson, R.: Peptides in the gut with a dual distribution in nerves and endocrine cells. In: Gut Hormones. S.R. Bloom (ed.), pp. 406–413. Edinburgh: Churchill-Livingstone 1978Google Scholar
  17. Tager, H.S.: Coupling of peptides to albumin with difluorodinitrobenzene. Anal. Biochem. 71, 367–375 (1976)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • I. Lorén
    • 1
  • J. Alumets
    • 1
  • R. Håkanson
    • 2
  • F. Sundler
    • 1
  • J. Thorell
    • 3
  1. 1.Department of HistologyUniversity of LundLundSweden
  2. 2.Department of PharmacologyUniversity of LundLundSweden
  3. 3.Department of Nuclear MedicineMalmö General HospitalMalmöSweden

Personalised recommendations