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Histochemistry

, Volume 56, Issue 2, pp 147–154 | Cite as

Isolation and thin-layer chromatographic identification of several peptides with NH2-terminal tryptophan and their histochemical demonstration in the ACTH cells of the rat hypophysis

  • Seppo Partanen
Article

Summary

Methods for the isolation and thin-layer chromatographic identification of amino-terminal tryptophyl-peptides presumably responsible for histochemical tryptophyl-peptide reactions in the ACTH cells of the rat hypophysis are described. In the hypophyseal extract several tryptophylpeptide bands — depending on the homogenization solution — were demonstrated on thin-layer chromatograms. Tryptophyl-peptides were demonstrated from their fluorescence induced 1) with glyoxylic acid (glyoxylic acid introduced into the homogenization solution), 2) by exposure of the chromatographic plates to combined formaldehyde and chloral vapour or 3) by exposure to combined formaldehyde and acetyl chloride vapour. A positive PAS reaction was demonstrated in some tryptophyl-peptide bands. Thus, some tryptophyl-peptides seem to contribute to the observed PAS positivity of the ACTH cells.

Keywords

Public Health Peptide Chloride Formaldehyde Tryptophan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Seppo Partanen
    • 1
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland

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