, Volume 56, Issue 2, pp 147–154 | Cite as

Isolation and thin-layer chromatographic identification of several peptides with NH2-terminal tryptophan and their histochemical demonstration in the ACTH cells of the rat hypophysis

  • Seppo Partanen


Methods for the isolation and thin-layer chromatographic identification of amino-terminal tryptophyl-peptides presumably responsible for histochemical tryptophyl-peptide reactions in the ACTH cells of the rat hypophysis are described. In the hypophyseal extract several tryptophylpeptide bands — depending on the homogenization solution — were demonstrated on thin-layer chromatograms. Tryptophyl-peptides were demonstrated from their fluorescence induced 1) with glyoxylic acid (glyoxylic acid introduced into the homogenization solution), 2) by exposure of the chromatographic plates to combined formaldehyde and chloral vapour or 3) by exposure to combined formaldehyde and acetyl chloride vapour. A positive PAS reaction was demonstrated in some tryptophyl-peptide bands. Thus, some tryptophyl-peptides seem to contribute to the observed PAS positivity of the ACTH cells.


Public Health Peptide Chloride Formaldehyde Tryptophan 
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  1. Axelsson, S., Björklund, A., Falck, B., Lindvall, O., Svensson, L.-Å.: Glyoxylic acid condensation: a new fluorescence method for the histochemical demonstration of biogenic monoamines. Acta physiol. scand. 87, 57–62 (1973)Google Scholar
  2. Axelsson, S., Björklund, A., Lindvall, O.: Fluorescence histochemistry of biogenic monoamines. A study of the capacity of various carbonyl compounds to form fluorophores with biogenic monoamines in gas phase reactions. J. Histochem. Cytochem. 20, 435–444 (1972)Google Scholar
  3. Björklund, A., Håkanson, R., Lindvall, O., Sundler, F.: Fluorescence histochemical demonstration of peptides with NH2- or COOH-terminal tryptophan or dopa by condensation with glyoxylic acid. J. Histochem. Cytochem. 21, 253–265 (1973)Google Scholar
  4. Bloom, F., Battenberg, E., Rossier, J., Ling, N., Leppäluoto, J., Vargo, T.M., Guillemin, R.: Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis and anterior lobes of the pituitary gland, not in the neurophypophysis. Life Sci. 20, 43–48 (1977)Google Scholar
  5. Bowie, E.P., Williams, M.G., Rennels, E.G.: Evidence for PAS positive reaction of the corticotroph granules of the rat adenohypophysis. Histochemistry 38, 281–284 (1974)Google Scholar
  6. Cox, B.M., Gentleman, S., Su, T.-P., Goldstein, A.: Further characterization of morphine-like peptides (endorphins) from pituitary. Brain Res. 115, 285–296 (1976)Google Scholar
  7. Cox, B.M., Opheim, K.E., Teschemacher, H., Goldstein, A.: A peptide-like substance from pituitary that acts like morphine 2. Purification and properties. Life Sci. 16, 1777–1782 (1975)Google Scholar
  8. Eipper, B.A., Mains, R.E., Guenzi, D.: High molecular weight forms of adrenocorticotropic hormone are glycoproteins. J. Biol. Chem. 251, 4121–4126 (1976)Google Scholar
  9. Ewen, S.W.B., Rost, F.W.D.: The histochemical demonstration of catecholamines and tryptamines by acid- and aldehyde-induced fluorescence: microspectrofluorometric characterization of the fluorophores in models. Histochem. J. 4, 59–69 (1972)Google Scholar
  10. Håkanson, R., Larsson, L.-I., Nobin, A., Sundler, F.: Tryptamine or tryptophyl peptides in endocrine cells of the mammalian adenohypophysis. J. Histochem. Cytochem. 20, 908–916 (1972)Google Scholar
  11. Håkanson, R., Sundler, F., Larsson, L.-I., Ekman, R., Sjöberg, N.-O.: Peptides with NH2-terminal tryptophan in adrenocorticotrophic hormone and melanocyte-stimulating hormone granules of adenohypophysis. J. Histochem. Cytochem. 23, 65–74 (1975)Google Scholar
  12. Håkanson, R., Sundler, F., Nobin, A., Sjöberg, N.-O., Edvinsson, L., Larsson, L.-I.: Peptides with NH2-terminal tryptophan in the adenohypophysis. Cell Tissue Res. 150, 281–290 (1974)Google Scholar
  13. Iturriza, F.C., Zicher, L.M., Ruager, J.: Detection of tryptamine in the pars distalis of the pituitary gland of the sheep. Acta endocr. panam. 3, 121–127 (1972)Google Scholar
  14. Jonsson, G.: The Formaldehyde Fluorescence Method for the Histochemical Demonstration of Biogenic Monoamines. A Methodological study. Stockholm: Ivar Heggströms Tryckeri Ab 1967Google Scholar
  15. Ling, N., Burgus, R., Guillemin, R.: Isolation, primary structure, and synthesis of α-endorphin and γ-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity. Proc. Natl. Acad. Sci. USA 73, 3942–3946 (1976)Google Scholar
  16. Mains, R.E., Eipper, B.A., Ling, N.: Common precursor to corticotrophins and endorphins. Proc. Natl. Acad. Sci. USA 74, 3014–3018 (1977)Google Scholar
  17. Partanen, S.: Fluorescence histochemistry of biogenic amines, tryptophan and tryptophyl-peptides in the developing hypophysis of the rat and the human fetus. Acta Inst. Anat. Univ. Helsinki., Suppl. 10 (1975a)Google Scholar
  18. Partanen, S.: Simultaneous fluorescence histochemical demonstration of catecholamines and tryptophyl-peptides in endocrine cells. Histochemistry 43, 295–303 (1975b)Google Scholar
  19. Partanen, S.: Fluorophore formation of tryptophyl-peptide in histofluorescence methods using carbonyl compounds. Proc. Fifth Int. Congr. Histochem. Cytochem. Bucharest, p. 269 (1976a)Google Scholar
  20. Partanen, S.: Reactivity of tissue tryptophyl units in histofluorescence methods using carbonyl compounds. Histochemistry 47, 339–343 (1976b)Google Scholar
  21. Partanen, S.: Carbonyl compound-induced fluorescence of biogenic monoamines in the endocrine cells of the hypophysis. Prog. Histochem. Cytochem. 10/3 (1978)Google Scholar
  22. Partanen, S., Rechardt, L., Bäck, N.: Combined formaldehyde and acetylchloride vapour condensation: a new fluorescence histochemical method for the demonstration of tryptophyl-peptide-containing endocrine cells of the hypophysis. Histochemistry 41, 119–131 (1974)Google Scholar
  23. Pearse, A.G.E., MacGregor, M.M.: Functional cytology of the pituitary gland. Ann. Rep. Brit. Emp. Cancer Camp. 2, 665–666 (1964)Google Scholar
  24. Pearse, A.G.E., van Noorden, S.: The functional cytology of the human adenohypophysis. Canad. Med. Ass. J. 88, 462–471 (1963)Google Scholar
  25. Rubinstein, M., Stein, S., Gerber, L.D., Udenfriend, S.: Isolation and characterization of the opioid peptides from rat pituitary: β-Lipotropin. Proc. Natl. Acad. Sci. USA 74, 3052–3055 (1977)Google Scholar
  26. Smith, I. (Ed.): Chromatographic and Electrophoretic Techniques. Vol. 1 & 2. 3. Ed. London: William Heinemann 1969Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Seppo Partanen
    • 1
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland

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