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Histochemistry

, Volume 71, Issue 4, pp 599–607 | Cite as

Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle

III. A modified simultaneous coupling technique
  • P. J. Stoward
  • B. Al-Sarraj
Article

Summary

Meijer's semipermeable membrane technique for acid phosphatase was modified by treating sections immediately after incubation with 70% ethanol for 30 min at room temperature instead of with formalin. The modification improved the validity of the technique considerably, by stabilising the specific final reaction product formed in presumed enzyme-containing sites. The modified technique thus seems promising for assaying the activity of acid phosphatase in sections of skeletal muscle.

Keywords

Public Health Skeletal Muscle Formalin Acid Phosphatase Final Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Christie KN, Stoward PJ (1977) A cytochemical study of acid phosphatase in dystrophic hamster muscle. J. Ultrastruct Res 58:219–234Google Scholar
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  4. Stoward PJ (1980) Criteria for the validation of quantitative histochemical techniques. In: Trends in “Enzyme histochemistry and cytochemistry” (Ciba Foundation Symposium 73). Excerpta Media, AmsterdamGoogle Scholar
  5. Stoward, PJ, Al-Sarraj B (1981) Quantitative histochemical investigations of the semipermeable membrane technique for the assay of acid phosphatase in skeletal muscle. I. Meijer's method. Histochemistry 71:405–431Google Scholar
  6. Stoward PJ, Al-Sarraj B, Ireland H (1981) Quantitative histochemical investigations of the semipermeable membrane technique for the assay of acid phosphatase in skeletal muscle. II. Non-specific reactions in control sections. Histochemistry 71:585–598Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • P. J. Stoward
    • 1
  • B. Al-Sarraj
    • 1
  1. 1.Department of AnatomyUniversity of DundeeDundeeScotland

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