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Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle

III. A modified simultaneous coupling technique

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Summary

Meijer's semipermeable membrane technique for acid phosphatase was modified by treating sections immediately after incubation with 70% ethanol for 30 min at room temperature instead of with formalin. The modification improved the validity of the technique considerably, by stabilising the specific final reaction product formed in presumed enzyme-containing sites. The modified technique thus seems promising for assaying the activity of acid phosphatase in sections of skeletal muscle.

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Authors and Affiliations

  1. Department of Anatomy, University of Dundee, DD1 4HN, Dundee, Scotland

    P. J. Stoward & B. Al-Sarraj

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  1. P. J. Stoward
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  2. B. Al-Sarraj
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Stoward, P.J., Al-Sarraj, B. Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. Histochemistry 71, 599–607 (1981). https://doi.org/10.1007/BF00508385

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  • DOI: https://doi.org/10.1007/BF00508385

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