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A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin a and periodic acid-Schiff

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Summary

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color α-d-glucosyl and α-d-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

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  1. Laboratory of Histology, Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

    K. Yamada, H. Kitamura & Y. Maseki

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  1. K. Yamada
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  2. H. Kitamura
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  3. Y. Maseki
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Yamada, K., Kitamura, H. & Maseki, Y. A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin a and periodic acid-Schiff. Histochemistry 71, 513–520 (1981). https://doi.org/10.1007/BF00508377

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  • DOI: https://doi.org/10.1007/BF00508377

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