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The formaldehyde-induced fluorescence of the developing hypogastric (main belvic) ganglion of the rat

Short adrenergic neurons and the effect of testosterone

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Summary

The development of the hypogastric ganglion of normal and testosterone-treated rats was studied using formaldehyde-induced fluorescence (FIF) method. The fluorescence intensities were recorded microspectrofluorimetrically. In normally developing rats cytoplasmic FIF decreases and cell size increases with age. In testosterone-treated animals FIF increases during 2–6 weeks compared to the controls. The differences between control and experimental rats were significant. The diameters were significantly longer in treated animals in three and four week old groups. Vacuolated neurons were seen earlier in testosterone-treated rats. No changes in FIF or in cell size were noticed in the superior cervical ganglion. The male sex steroid, testosterone evidently influences the catecholamine turnover and cellular growth during development in the male pelvic ganglion.

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References

  • Alho, H., Partanen, M., Hervonen, A.: Microspectrofluorimetric quentitation of catecholamine fluorescence in the rat sympathetic ganglia. II. The effect of hydrocortixone and testosterone in the superior cervical ganglion at different ages. (Manuscript, to be published 1979)

  • Becker, K.: Über die vakuolenhaltigen Nervenzellen im Ganglion cervicale uteri der Ratte. Z. Zellforsch. 68, 318–339 (1968)

    Google Scholar 

  • Broberg, A., Nubell, G., Owman, Ch., Rosengren, E., Sjöberg, N.-O.: Consequence of Neonatal Androgenization and Castration for Future Levels of Norepinephrine Transmitter in Uterus and Vas Deferens of the Rat. Neuroendocrinology 15, 308–312 (1974)

    Google Scholar 

  • Champlain, J. de, Malmfors, T., Oslon, L., Sachs, C.: Ontogenesis of peripheral adrenergic neurons in the rat: pre- and postnatal observations. Acta Physiol Scand. 80, 276–288 (1970)

    Google Scholar 

  • Dail, W.G., Jr., Evan, A., Jr., Eason, H.R.: The major ganglion in the pelvic plexus of the male rat. A histochemical study and ultrastructural study. Cell Tissue Res. 159, 49–62 (1975)

    Google Scholar 

  • Eränkö, L.: Postnatal development of histochemically demonstratable catecholamines in superior cervical ganglion of the rat. Histochem. J. 4, 225–236 (1972)

    Google Scholar 

  • Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. R. Microsc. Soc. 87, 259–276 (1967)

    Google Scholar 

  • Falck, B., Owman, Ch., Rosengren, E., Sjöberg, N.-O.: Persisting high level of transmitter in uterine short adrenergic neurons following prolonged treatment with 17β-oestradiol. Acta Endocrinol. 62, 77–81 (1969)

    Google Scholar 

  • Fernholm, M.: On the appearance of monoamines in the sympathetic systems and the chromaffin tissue in the mouse embryo. J. Anat. Entwicklungsgesch. 135, 350–361 (1972)

    Google Scholar 

  • Greenberg, S., Long, J.P., Burke, J.P., Chapnick, B., Orden, L.S. van: III Decreased contractility and norepinephrine content of guinea-pig seminal vesicles after chronic treatment with testosterone. J. Pharmacol. Exp. Ther. 184, 56–66 (1975)

    Google Scholar 

  • Hervonen, A., Kanerva, L., Lietzen, R., Partanen, S.: Ultrastructural Changes Induced by Estrogen in the Adrenergic Nerves of the Rabbit Myometrium. Acta Physiol. Scand. 85, 283–285 (1972)

    Google Scholar 

  • Hervonen, A., Kanerva, L.: Effect of 17 β-estradiol on the axoplasmic organelles of the adrenergic axons of the rabbit myometrium, as revealed after KMnO4-fixation. Z. Zellforsch. 144, 219–229 (1973)

    Google Scholar 

  • Hervonen, A., Partanen, M., Vaalasti, A., Kanerva, L.: The effect of androgenisation on the development of the adrenergic innervation of the male accessory genital glands and the hypogastric ganglion (main pelvic ganglion) of the rat. Xth Acta Endocrinologica Congress, Amsterdam (1975)

  • Hervonen, A., Vaalasti, A., Partanen, M., Kanerva, L., Hervonen, H.: Effects of ageing on the histochemically demonstrable catecholamines and acetylcholinesterase of human sympathetic ganglia. J. Neurocytol. 7, 11–23 (1978)

    Google Scholar 

  • Kanerva, L.: The postnatal development of monoamines and cholinesterases in the paracervical ganglion of the rat uterus. Prog. Brain Res. 34, 433–444 (1971)

    Google Scholar 

  • Kanerva, L., Teräväinen, H.: Electron microscopy of the paracervical (Frankenhäuser) Ganglion of the adult Rat. Z. Zellforsch. 129, 161–177 (1972)

    Google Scholar 

  • Kanerva, L., Lietzen, Teräväinen, H.: Catecholamines and cholinesterases in the Paracervical (Frankenhäuser) Ganglion of Normal and Pregnant Rats. Acta Physiol. Scand. 86, 271–277 (1972)

    Google Scholar 

  • Kanerva, L., Hervonen, A., Hervonen, H.: Morphological characteristics of the ontogenesis of the mammalian peripheral adrenergic nervous system with special remarks on the human fetus. Med. Biol. 52, 144–152 (1974)

    Google Scholar 

  • Kvist, U., Sjöstrand, N.-O.: A Further Contribution to the Question of Trophic and Hormonal Influences on the Noradrenaline Content of the Male Reproductive Tract: Effect of Combined Androgen and Estrogen Treatment of Prepuberally Castrated Rats. Acta Physiol. Scand. 98, 339–346 (1976)

    Google Scholar 

  • Lehman, H.J., Stange, H.H.: Über das Vorkommen vakuolen-haltiger Ganglienzellen im Ganglion cervicale uteri trächtiger und nichtträchtiger Ratten. Z. Zellforsch. 38, 230–236 (1953)

    Google Scholar 

  • Owman, Ch., Sjöberg, N.-O., Sjöstrand, N.-O.: Short adrenergic neurons, a peripheral neuroendocrine mechanism. In: Amine fluorescence histochemistry. Eds. M. Fujiwara, C. Tanaka (eds.), pp. 47–66. Tokyo: Igaku Shoin 1974

    Google Scholar 

  • Partanen, M., Hervonen, A., Vaalasti, A., Kanerva, L.: Neuroendocrine observations on the development of the hypogastric ganglion of the rat. The 5th International Congress of Histochemistry and Cytochemistry, Bukarest (1976).

  • Partanen, M., Hervonen, A., Vaalasti, A., Kanerva, L. Hervonen, H.: Vacuolated neurons in the hypogastric ganglion of the rat. Cell Tissue Res. in press (1979)

  • Pawlikowski, M.: Das Ganglion prostaticum und das Ganglion cervicale superius normaler und gonadopriver Ratten. (Poln.) Endokrynol. Pol. 10, 449–458 (1959)

    Google Scholar 

  • Pawlikowski, M.: The occurrence of vacuoles in the nerve cells of autonomic ganglia as a sign of neurosecretion. Polish. Med. Sci. Hist. Bull. 4, 110–112 (1961)

    Google Scholar 

  • Pawlikowski, M.: Studies on peripheral neurosecretion. I. Morphological and topographic features of neurosecretion in mammalian autonomic ganglions. Endokrynol. Pol. 13, 153–170 (1962a)

    Google Scholar 

  • Pawlikowski, M.: The effect of gonadal and gonadotrophic hormones on the prostatic ganglion and the superior cervical ganglion in male rats. Acta Med. Pol. 3, 171–183 (1962b)

    Google Scholar 

  • Ryd, G., Sjöstrand, N.-O.: Effect of Castration and Testosterone administration on the Noradrenaline content of the Vas Deferens and Seminal Vesicle of the Guinea-pig. Experientia 23, 816–817 (1967)

    Google Scholar 

  • Sjöberg, N.-O.: Increase in transmitter content of adrenergic nerves in the reproductive tract of female rabbits after oestrogen treatment. Acta Endocrinol. (Kbh.) 57, 405–413 (1968)

    Google Scholar 

  • Sjöstrand, N.-O., Swedin, G.: Influence of Age, Growth, Castration and Testosterone Treatment on the Noradrenaline evels of the Ductus Deferens and the Auxiliary Male Reproductive Glands of the Rat. Acta Physiol. Scand. 98, 323–338 (1967)

    Google Scholar 

  • Swedin, G.: Studies on neurotransmission mechanism in the rat and guinea-pig vas deferens. Acta Physiol. Scand. Suppl. 369, (1971)

  • Stange, H.H., Drescher, I.: Tierexperimentelle Untersuchungen am Frakenhäuserschen Ganglion zum Problem der peripheren Neurosekretion. Arch. Gynäkol. 184, 530–542 (1954)

    Google Scholar 

  • Takahashi, O.: On the formation of vacuoles in the nerve cells of the ganglion cervicalis uteri on the rat and mouse. Okajimas Folia Anat. Jpn. 34, 189–200 (1960)

    Google Scholar 

  • Wakade, A.R., Kirpekar, S.M.: “Trophic” influence on the sympathetic nerves of the vas deferens and seminal vesicle of the guinea-pig. J. Pharmacol. Exp. Ther. 186, 528–536 (1973)

    Google Scholar 

  • Wakade, A.R., Garcia, A.G., Kirpekar, S.M.: Effect of castration on the smooth muscle cells of the internal sex organs of the rat: influence of the smooth muscle on the sympathetic neurons innervating the vas deferens, seminal vesicle and coagulating gland. J. Pharmacol. Exp. Ther. 193, 424–434 (1975)

    Google Scholar 

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  1. Department of Biomedical Sciences, University of Tampere, Box 607, SF-33101, Tampere 10, Finland

    M. Partanen & A. Hervonen

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  1. M. Partanen
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  2. A. Hervonen
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Partanen, M., Hervonen, A. The formaldehyde-induced fluorescence of the developing hypogastric (main belvic) ganglion of the rat. Histochemistry 62, 249–258 (1979). https://doi.org/10.1007/BF00508353

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