Summary
The influence of the concentrations of NaCl, NaJ, KJ and/or Na2SO4 on the foaminess of BSA solutions is investigated. The foaminess increases with increasing salt concentrations as expected for NaCl, NaJ and Na2SO4. With KJ the foaminess exhibits an anomaly. The dependence of the foaminess on the pH is complex. In the presence of buffer there is a minimum at 4.81 and a maximum at 4.7. In the absence of buffer the foaminess reaches a maximum at pH 4 and a minimum at 3. The anomaly of BSA solutions is well-known but not yet fully understood.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- BSA:
-
Bovine Serum Albumin
- C:
-
concentration
- CBSA :
-
concentration of BSA
- CBUFFER :
-
concentration of buffer
- CSALT :
-
concentration of salt
- Vs :
-
equilibrium volume of the foam
- Vtg :
-
volumetric gas flow rate
- Σ=Vs/Vtg :
-
foaminess
References
Bikerman JJ (1973) Foams. Berlin, Heidelberg, New York, Springer
Bumbullis W, Kalischewski K, Schügerl K (1979) Foam Behavior of Biological Media II. Europ. J. Appl. Microbiol. Biotechnol. 7, 147–154
Cumper CWN (1953) The Stabilisation of Foams by Proteins. Trans. Farad. Soc. 49, 1360–1369
Radola BJ (1973) Isoelectric Focusing in Layers of Granulated Gels. I. Thin Layer Isoelectric Focusing of Proteins. Biochim. Biophys. Acta 295, 412–428
Saroff HA, Loeb GI, Scheraga HA (1955) Low and High pH Effects in Serum Albumin. J. Am. Chem. Soc. 77, 2908–2910
Tanford Ch, Buzzell JG (1955) The Reversible Expansion of Bovine Serum Albumin in Acid Solutions. J. Am. Chem. Soc. 77, 6421–6428
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kotsaridu, M., Gehle, R. & Schügerl, K. Foam behavior of biological media. European J. Appl. Microbiol. Biotechnol. 18, 60–63 (1983). https://doi.org/10.1007/BF00508130
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00508130