Summary
The subcellular localization of glucose oxidase (E.C. 1.1.3.4) in mycelia of Aspergillus niger has been investigated using cytochemical staining techniques. Mycelia from fermenter cultures, which produced gluconic acid from glucose, contained elevated levels of glucose oxidase and catalase. Both enzymes were located in microbodies. In addition, when the organism was grown on glucose with methylamine as a nitrogen source, amine oxidase activity was detected in the microbodies. These organelles can therefore be designated as peroxisomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avers CJ (1971) Sub-Cell Biochem 1:25–37
Bentley R (1963) In: Boyer PD, Hardy H. Myrbäck K (eds) The enzymes, 2nd edn vol VII. Academic Press, New York, London, p 567
Buchholz K, Gödelmann B (1978) Biotechnol Bioeng 20:1201–1220
Dijken JP van, Veenhuis M, Kreger-van Rij NJW, Harder W (1975) Arch Microbiol 102:41–44
Duve C, De Baudhuin P (1966) Physiol Rev 46:323–357
Fukui S, Kawamoto S, Yasuhara S, Tanaka A, Osumi M, Imaizumi F (1975) Eur J Biochem 59:561–566
Gibson QH, Swoboda BEP, Massey V (1964) J Biol Chem 240:2209–2215
Harder W, Visser K, Kuenen JG (1974) Lab Pract 23:644–645
Hoffmann HP, Szabo A, Avers CJ (1970) J Bacteriol 104:581–584
Keilin D, Hartree EF (1948) Biochem J 42:221–229
Kusai K, Sekuzu I, Hagihara B, Okunuki K, Yamauchi S, Nakai M (1960) BBA 40:557–559
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275
Lück H (1963) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, London p 885
Masters C, Holmes R (1977) Physiol Rev 57:816–882
Maxwell DP, Armentrout VN, Graves LB Jr (1977) Ann Rev Phytopathol 15:119–134
Pazur JH (1966) Glucose oxidase. In: Cowolick SP, Kaplan NO (eds) Methods in enzymol IX. Academic Press, New York, London p 83
Pazur JH, Kleppe K (1964) Biochem 3:578–583
Roggenkamp R, Sahm H, Hinkelman W, Wagner F (1975) Eur J Biochem 59:231–236
Sahm H, Roggenkamp R, Wagner F, Hinkelman W (1975) J Gen Microbiol 88:218–222
Spurr AR (1969) J Ultrastruct Res 26:31–46
Tanaka A, Yasuhara S, Gellf G, Osumi M, Fukui S (1978) Eur J Appl Microbiol Biotechnol 5:17–27
Veenhuis M, Dijken JP van, Harder W (1976) Arch Microbiol 111:123–135
Yamada H, Adachi O, Ogata K (1965a) Agric Biol Chem 29:117–123
Yamada H, Adachi O, Ogata K (1965b) Agric Biol Chem 29:649–654
Zwart K, Veenhuis M, Dijken JP van, Harder W (1980) Arch Microbiol, in press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Dijken, J.P., Veenhuis, M. Cytochemical localization of glucose oxidase in peroxisomes of Aspergillus niger . European J. Appl. Microbiol. Biotechnol. 9, 275–283 (1980). https://doi.org/10.1007/BF00508104
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00508104