Physiology of α-amylase production by immobilized Bacillus amyloliquefaciens
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Bacillus amyloliquefaciens 321S cells were immobilized with 3.4% κ-carrageenan gel in bead form, and α-amylase production by the immobilized cells was studied. Cells in the gel, after the population reached maximum were restricted to a layer of 50 μm thickness, from the surface of the gel, suggesting that oxygen diffusion is the growth limiting factor. The specific respiratory activity and the growth rate of the entrapped cells under such conditions were 1/2 and 1/5 ∼1/10, respectively, that of free cells. In spite of the repressed respiration and growth, the specific rate of α-amylase production of the entrapped cells reached the maximum value of free cells or higher.
In continuous culture, in an aerated vessel with a volume ratio of gel beads to medium of 1:2, the maximum production rate of α-amylase was obtained at a dilution rate of 1.0 h−1, which was double the maximum specific growth rate of the strain.
These results showed that bacterial α-amylase production, which is a nongrowth-associated type of synthesis was achieved with the use of immobilized cells.
KeywordsRespiration Bacillus Specific Growth Rate Dilution Rate Immobilize Cell
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