Histochemistry

, Volume 71, Issue 2, pp 187–194 | Cite as

The use of a carbodiimide-containing fixative for the immunohistochemical demonstration of coagulation factor VIII in rat vascular tissue

  • E. van Pelt-Verkuil
  • J. J. Emeis
Article

Summary

Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity. Carbodiimide is preferable as a fixative for the cellular localization of F-VIII-R:AG.

Keywords

Public Health Endothelial Cell Aldehyde Factor VIII Vascular Endothelial Cell 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • E. van Pelt-Verkuil
    • 1
  • J. J. Emeis
    • 1
  1. 1.Gaubius InstituteHealth Research Organization TNOLeidenThe Netherlands

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