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Histochemistry

, Volume 57, Issue 1, pp 93–96 | Cite as

A modified wire-loop method for quantitative electron microscopic radioautography

  • K. Yoshida
  • F. Murata
  • S. Ohno
  • T. Nagata
Short Communication

Summary

A simplified method of emulsion coating and development for quantitative electron microscopic radioautography without any special instrument is introduced.

Seven grids carrying ultrathin sections are attached to a glass block with double-coated Scotch tape. They are coated with a wire-loop, 2.5 cm in diameter. Ten glass blocks are then stuck on a slide, exposed, developed, fixed and stained simultaneously.

Employing this method, the emulsion coating and the following procedures are easily accomplished under an identical condition.

Keywords

Public Health Identical Condition Ultrathin Section Special Instrument Scotch Tape 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Caro, L.G., van Tubergen, R.P.: High resolution autoradiography. I. Methods. J. Cell Biol. 15, 173–188 (1962)Google Scholar
  2. Kopriwa, B.M.: A reliable, standardized method for ultrastructural electron microscopic radioautography. Histochemie 37, 1–17 (1973)Google Scholar
  3. Nagata, T., Nawa, T.: A modification of dry-mounting technique for radioautography of watersoluble compounds. Histochemie 7, 370–371 (1966)Google Scholar
  4. Nagata, T., Nawa, T., Yokota, S.: A new technique for electron microscopic dry-mounting radioautography of soluble compounds. Histochemie 18, 241–249 (1969)Google Scholar
  5. Nagata, T.: A simplified method for mass production of electron microscopic radioautographs. Acta histochem. cytochem. 6, 61 (1973)Google Scholar
  6. Salpeter, M.M., Bachmann, L.: Autoradiography with the electron microscope. A procedure for improving resolution, sensitivity, and contrast. J. Cell Biol. 22, 469–477 (1964)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • K. Yoshida
    • 1
  • F. Murata
    • 1
  • S. Ohno
    • 1
  • T. Nagata
    • 1
  1. 1.Department of AnatomyShinshu University School of MedicineMatsumotoJapan

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