Skip to main content
SpringerLink
Log in

A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

A comparison between the one-step and the two-step copper thiocholine procedure for the subcellular localization of cholinesterases was under-taken. The results indicate that only under experimental conditions with short incubation times and precise control of the conversion into sulphide is the localization of the primary precipitate and that of the secondary precipitate identical. It was concluded that the conversion of the primary precipitate into Cu-sulphide is not necessary and can lead to artefacts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Brzin, M., Tennyson, V.M., Dettbarn, W.D.: Cytochemical localization of cholinesterase activity at the giant synapse of the Squid. Histochemistry 43, 305–311 (1975)

    Google Scholar 

  • Brzin, M., Tennyson, V.M., Duffy, P.E.: Acetylcholinesterase in frog sympathetic and dorsal root ganglia. A study by electron microscope cytochemistry and microgasometric analysis with magnetic diver. J. Cell Biol. 31, 215–242 (1966)

    Google Scholar 

  • Holmstedt, B.: A modification of the thiocholine method for the demonstration of cholinesterase. I. Biochemical evaluation of selective inhibitors. Acta physiol. scand. 25, Suppl. 90, 322–330 (1957)

    Google Scholar 

  • Koelle, G.B., Friedenwald, J.S.: A histochemical method for localizing cholinesterase activity. Proc. Soc. exp. Biol. (N.Y.) 70, 617–622 (1949)

    Google Scholar 

  • Lewis, P.R., Shute, C.C.D.: Electronmicroscopic study of cholinesterase distribution in the rat adrenal medula. J. Microsc. (Oxford) 89, 181–193 (1969)

    Google Scholar 

  • Mohr, E., Gerebtzoff, M.A.: Récherches histochimiques sur les acetylcholine et choline-esterases. Acta anat. (Basel) 22, 143–151 (1954)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electrone-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Smith, R.E., Farquhar, M.G.: Preparation of thick sections for cytochemistry and electron microscopy by a non-freezing technique. Nature (Lond.) 200, 691 (1963)

    Google Scholar 

  • Tennyson, V.M., Brzin, M., Slatwiner, P.: The appearance of acetylcholinesterase in the myotome of embrionic rabbit. An electron microscopic-cytochemical and biochemical study. J. Cell Biol. 51, 703–721 (1971)

    Google Scholar 

  • Zajićek, J., Sylvén, B., Datta, N.: Attempts to demonstrate acetylcholinesterase activity in blood and bone marrow cells by a modified thiocholine technique. J. Histochem. Cytochem. 2, 115–121 (1954)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathological Physiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 61000, Ljubljana, Yugoslavia

    Biba Klinar & Miro Brzin

Authors
  1. Biba Klinar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Miro Brzin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klinar, B., Brzin, M. A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases. Histochemistry 50, 313–318 (1977). https://doi.org/10.1007/BF00507124

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00507124

Keywords

Search

Navigation