Summary
The injection of rabbits with insoluble or soluble G-actin from chicken smooth or striated muscle will produce antibodies that are equally reactive, and species and tissue non-specific in immunoprecipitation, immunofluorescence and actin-activated Mg2+-ATPase** inhibition test. These antibodies have been used for the identification of actin-containing fibrils in a variety of tissues. When G-actins from chicken smooth or striated muscle are immobilized by chemical linkage to Affi-Gel 702 microbeads, their immunogenicity is increased, but the antibodies obtained against them are species-specific and will only react with actin and actin-containing structures from chicken and are therefore limited in use. It is concluded from this work that insoluble G-actin is the preferable immunogen to obtain precipitating antibodies for wide use.
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Abbreviations
- ATPase:
-
Adenosinetriphosphatase
- FITC:
-
Fluoresceinisothiocyanate
- SDS:
-
Sodiumdodecylsulfate
References
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This paper is dedicated to Dr. Dorothy M. Needham, University of Cambridge, England, in honour of her eightieth birthday
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Gröschel-Stewart, U., Ceurremans, S., Lehr, I. et al. Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. Histochemistry 50, 271–279 (1977). https://doi.org/10.1007/BF00507120
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DOI: https://doi.org/10.1007/BF00507120