, Volume 50, Issue 4, pp 271–279 | Cite as

Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy

II. Species-specific and species-non-specific antibodies to smooth and striated chicken muscle actin
  • Ute Gröschel-Stewart
  • Susi Ceurremans
  • Irmtraut Lehr
  • Christine Mahlmeister
  • Eltriede Paar


The injection of rabbits with insoluble or soluble G-actin from chicken smooth or striated muscle will produce antibodies that are equally reactive, and species and tissue non-specific in immunoprecipitation, immunofluorescence and actin-activated Mg2+-ATPase** inhibition test. These antibodies have been used for the identification of actin-containing fibrils in a variety of tissues. When G-actins from chicken smooth or striated muscle are immobilized by chemical linkage to Affi-Gel 702 microbeads, their immunogenicity is increased, but the antibodies obtained against them are species-specific and will only react with actin and actin-containing structures from chicken and are therefore limited in use. It is concluded from this work that insoluble G-actin is the preferable immunogen to obtain precipitating antibodies for wide use.


Public Health Striate Muscle Fibril Specific Antibody Immunofluorescence Microscopy 
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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Ute Gröschel-Stewart
    • 1
  • Susi Ceurremans
    • 1
  • Irmtraut Lehr
    • 1
  • Christine Mahlmeister
    • 1
  • Eltriede Paar
    • 1
  1. 1.Institut für Zoologie der Technischen Hochschule DarmstadtDarmstadtGermany

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