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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 306, Issue 3, pp 209–211 | Cite as

The affinity of atropine for muscarine receptors in human sphincter pupillae

  • A. J. Kaumann
  • R. Hennekes
Article

Summary

Concentration-dependent contractions in response to carbachol were determined on isolated pieces of human iris. Atropine competitively antagonized the effect of carbachol. An apparent dissociation equilibrium constant of 0.4–0.7 nM was estimated for the muscarine receptor-atropine complex.

Key words

Isolated human iris Carbachol Atropine Muscarine receptor 

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References

  1. Arunlakshana, O., Schild, H. O.: Some quantitative uses of drug antagonists. Br. J. Pharmacol. 14, 48–58 (1959)Google Scholar
  2. Barlow, R. B., Franks, F. M., Pearson, J. D. M.: A comparison of the affinities of antagonists for acetylcholine receptors in the ileum, bronchial muscle and iris of the guinea pig. Br. J. Pharmacol. 46, 300–314 (1972)Google Scholar
  3. Blinks, J. R.: Convenient apparatus for recording contractions of isolated muscle. J. Appl. Physiol. 20, 755–757 (1965)Google Scholar
  4. Duke-Elder, S.: The foundation of ophthalmology. Vol. VII, p. 543. (1962)Google Scholar
  5. Furchgott, R. F.: The use of β-haloalkylamines in the differentiation of receptors and in the determination of dissociation constants of receptor agonist complexes. Advances Drug Res 3, 21–55 (1966)Google Scholar
  6. Moses, R. A.: Adler's physiology of the eye. 6th ed., p. 348. Mosby (1975)Google Scholar
  7. Paton, W. D. M., Rang, H. P.: The uptake of atropine and related drugs by intestinal smooth muscle of the guinea pig in relation to acetylcholine receptors. Proc. R. Soc. Lond. [Biol.] 163, 1–44 (1965)Google Scholar
  8. Smith, S. A.: Factors determining the potency of mydriatic drugs in man. Br. J. Clin. Pharmacol. 3, 503–507 (1976)Google Scholar
  9. Stephenson, R. P.: A modification of receptor theory. Br. J. Pharmacol. 11, 379–393 (1956)Google Scholar
  10. Thron, C. D., Waud, D. R.: The rate of action of atropine. J. Pharmacol. Exp. Ther. 160, 91–105 (1968)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • A. J. Kaumann
    • 1
  • R. Hennekes
    • 2
  1. 1.Lehrstuhl für Klinische PhysiologiePhysiologisches Institut der UniversitätDüsseldorfFederal Republic of Germany
  2. 2.UniversitätsaugenklinikDüsseldorfFederal Republic of Germany

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