Advertisement

Histochemistry

, Volume 77, Issue 2, pp 285–288 | Cite as

Histochemical detection of thiosulphate sulphurtransferase (rhodanese) activity

  • D. Tanka
  • K. Gátai
Short Communications

Summary

A histochemical method is described by which the activity of thiosulphate sulphurtransferase (E.C. 2.8.1.1., rhodanese) can be detected. The method gives an easy and quick determination of enzyme activity. The basis of the detection is the reducing effect of sulphite (SO 3 2− ) ions resulting from the enzyme reaction. Nitro-blue tetrazolium chloride can be used as an indicator and N-methylphenazonium-methosulphate as an accelerator for increasing electron transport. — In mammals rhodanese is localized in the mitochondria.

Keywords

Public Health Chloride Enzyme Activity Electron Transport Enzyme Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. DeDuve CP, Pressmann BC, Gianetto R, Wattiaux R, Appelmans F (1955) Tissue fractionation studies 6. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem J 60:604–617Google Scholar
  2. Gátai K, Tanka D (1981) Role of collagenase and the cystathionase-rhodanese system in the pathogenesis of rheumathoid arthritis. VIII. European Congress of Pathology, Helsinki, Abstract: No 24Google Scholar
  3. Koj A, Frendo J, Wojtczak L (1975) Subcellular distribution and intramitochondrial localization of three sulphurtransferases in rat liver. FEBS Lett 57:42–46Google Scholar
  4. Lang K (1933a) Die Rhodanidebildung im Tierkörper. Biochem Z 259:243–256Google Scholar
  5. Lang K (1933b) Die Rhodanidebildung im Tierkörper. Biochem Z 263:262–267Google Scholar
  6. Ludewig S, Chanutin A (1950) Distribution of enzymes in the livers of controll and X-irradiated rats. Acta Biochem 29:441–445Google Scholar
  7. Sörbo BH (1953) Crystalline Rhodanese I. Purification and physico-chemical examination. Acta Chem Scand 7:1129–1136Google Scholar
  8. Sörbo BH (1975) Thiosulphate sulphurtransferase and mercaptopyruvate sulphurtransferase. In: Greenberg DM (ed) Metabolic pathways, vol. 7. Academic Press, New York London, pp 433–456Google Scholar
  9. Tanka D, Gátai K (1980a) Human collagenase and rhodanese: Activity and interaction of the enzymes from osteo-arthritic and rheumatoid synovium. Xth European Synposium on Osteo arthrology, MalmöGoogle Scholar
  10. Tanka D, Gátai K (1980b) The role of some sulphurtransferases in originating anti-collagen antibody. International Academy of Pathology Post Congress Meeting, Budapest, Abstract No 134Google Scholar
  11. Tanka D (1983) Rhodanese. Thyocyanat Monografie. Weuffen W (ed) (DDR)Google Scholar
  12. Westley J (1973) Rhodanese. Adv Enzymol 39:327–368Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • D. Tanka
    • 1
  • K. Gátai
    • 1
  1. 1.Department of PathologyNational Institute of Rheumatology and PhysiotherapyBudapest 114Hungary

Personalised recommendations