Histochemistry

, Volume 77, Issue 2, pp 159–170 | Cite as

Fluctuations in the enzymatic activity of the human endometrium

  • E. A. Elias
  • R. A. Elias
  • A. M. Kooistra
  • A. C. Roukema
  • J. F. Blacquiere
  • H. Barrowclough
  • A. E. F. H. Meijer
Article

Summary

Cyclic fluctuations were studied in the activity of oxidoreductases playing a role in the major energy metabolic pathways, lysosomal and non-lysosomal hydrolases and some non-enzymatic cytochemical components demonstrable in different developmental physiological or pathophysiological phases of human endometrium. The total scope of the study involved 170 tissues and cytological specimens. The cytological material included microcurettings, aspirates, brush preparations and tissue prints. An evaluation of the usefulness of the application of enzyme cytochemistry to cytological material is included. The most important results were a cyclic fluctuation and a progestagenic controlled increase in the activity of many oxidoreductases, especially the NADPH regenerating enzymes of the pentose phosphate pathway, and of the NADP+ dependent isocitrate dehydrogenase. The histochemical evaluation of the activity of these NADP+ linked enzymes can therefore be recommended for the evaluation of the physiological status of the endometrial cells, especially in patients with infertility problems.

Keywords

Infertility NADP Pentose Pentose Phosphate Pathway Isocitrate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aschheim S (1915) Zur Histologie der Uterusschleimhaut. Über das Vorkommen von Fettsubstanzen. Z Geburtsh Gynäkol 77:485–496Google Scholar
  2. Barka MD, Anderson PJ (1963) Histochemistry, theory, practice and bibliography. Harper and Row, New YorkGoogle Scholar
  3. Bitensky L, Cohen S (1965) The variation of endometrial acid phosphatase activity with the menstrual cycle. J Obstet Gynaecol Br Commun 72:769–774Google Scholar
  4. Black J, Heyns OS, Gillman J (1941) The value of basal fat in the human uterus as an indicator of optimum progesterone activity. J Clin Endocrinol 1:547–553Google Scholar
  5. Coulton LA (1977) Temporal relationship between glucose-6-phosphate dehydrogenase activity and DNA-synthesis. Histochemistry 50:207–215Google Scholar
  6. Craig JM, Danziger S (1965) Histological distribution and nature of stainable lipids of the human endometrium. Am J Obstet 93:1018–1023Google Scholar
  7. Dallenbach-Hellweg G (1981) Histopathology of the endometrium. Springer, Berlin Heidelberg New YorkGoogle Scholar
  8. Elias EA, Elias RA, v d Baan R (1981) Histochemical and cytochemical studies on metastasizing carcinoma of Bartolin's gland. Acta Histochem 69:31–39Google Scholar
  9. Elias EA, Elias RA, Bijlsma PJ, Tazelaar J (1980) The enzyme histochemistry of metastasizing basal cell carcinoma of the skin. J Pathol 131:235–241Google Scholar
  10. Elias EA, Meijer AEFH (1981) The increase in activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in skeletal muscles of rats after subcutaneous administration of N,N-dimethyl-paraphenylenediamine. Histochemistry 71:543–558Google Scholar
  11. Elias EA, Elias RA, de Vries GP, Meijer AEFH (1982) Early and late changes in the metabolic pattern of the working myocardial fibres and Purkinje fibres of the human heart under ischemic and inflammatory conditions. Histochem J 14:445–459Google Scholar
  12. Ferenczy A, Richert RM, Agathe FJ, Pukerson ML, Dempsy EW (1972) Scanning electron microscopy of human heart endometria surface epithelium. Fertil Steril 23:515–521Google Scholar
  13. Froboese C (1924) Verfehlung des Endometriums. Beitrag zur normalen und pathologischen Anatomie der Uterusschleimhaut. Virchows Arch Pathol Anat 250:296–342Google Scholar
  14. Fuhrmann K (1961) Hormone and Fermente des Endometriums. Gynaecologia (Basel) 152:1–22Google Scholar
  15. Goebell H, Kadenbach B (1964) DPN- und TPN-spezifische Isocitrat-Dehydrogenase als Indikatorenzyme für die oxydativen und synthetischen Stoffwechselwege. Verh Dtsch Ges Inn Med 70:665–668Google Scholar
  16. Henderson JF (1972) Regulation of purine biosynthesis. Am Chem Soc Monogr 170Google Scholar
  17. Kapuscinski M, Williams JF (1981) There is an active and extensive pentose phosphate pathway of glucose metabolism in rat heart. J Mol Cell Cardiol 13 (Suppl 3):4Google Scholar
  18. Koudstaal J (1975) The histochemical demonstration of arylsulphatase in human tumours. Eur J Cancer 11:809–813Google Scholar
  19. Lardy HA, Paetkau W, Walter P (1965) Path of carbon in gluconeogenesis and lipogenesis. The role of mitochondria in supplying precursors of phosphoenolpyruvate. Proc Natl Acad Sci USA 53:1410–1415Google Scholar
  20. Liebig W, Stenger HE (1977) Dezidualization der endometrialen Stromazellen. E.M. Untersuchungen. Arch Gynaekol 223:19–31Google Scholar
  21. Lojda Z, Gossrau R, Schiebler TH, (1979) Enzyme histochemistry. Springer, Berlin Heidelberg New YorkGoogle Scholar
  22. Meijer AEFH (1972) Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. I. Acid phosphatase. Histochemie 30:31–39Google Scholar
  23. Meijer AEFH (1973) Semipermeable membranes for improving the histochemical demonstration of enzyme activites in tissue sections. III. Lactate dehydrogenase. Histochemie 35:165–172Google Scholar
  24. Meijer AEFH (1978) Adaptations of methods in enzyme histochemistry to the different metabolic conditions in cells and tissues. Cell Mol Biol 23:159–170Google Scholar
  25. Meijer AEFH (1980) Semipermeable membrane techniques in quantitative enzyme histochemistry. In: Trends in enzyme histochemistry and cytochemistry, (Ciba foundation, symposium 73, pp 103–120). Excerpta Medica, North Holland, AmsterdamGoogle Scholar
  26. Meijer AEFH, Elias EA (1977) Die Aktivität der Glucose-6-Phosphat-Dehydrogenase in Skelettmuskelgewebe von Patienten mit Muskelkrankheiten. Acta Histochem (Suppl) 18:169–175Google Scholar
  27. Meijer AEFH, Elias EA (1983) Manuscript in preparationGoogle Scholar
  28. Meijer AEFH, Vloedman AHT (1973) Semipermeable membranes for improving the histochemical demonstration of enzyme activiities in tissue sections. II. Non specific esterases and β-glucuronidase. Histochemie 34:127–134Google Scholar
  29. Meijer AEFH, de Vries GP (1974) Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. IV. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating). Histochemistry 40:349–359Google Scholar
  30. Meijer AEFH, de Vries GP (1975) Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. V. Isocitrate: NADP+ oxidoreductase (decarboxylating) and malate: NADP+ oxidoreductase (decarboxylating). Histochemistry 43:225–236Google Scholar
  31. Meijer AEFH, Elias EA, Vloedman AHT (1977) The value of enzyme histochemical techniques in classifying fibre types of human skeletal muscle. III. Human skeletal muscles with inherited or acquired disease of the neuromuscular system. Histochemistry 53:97–105Google Scholar
  32. Pearse AGE (1972) Histochemistry. Theoretical and applied, vol 2. 2nd ed. Churchill, LondonGoogle Scholar
  33. Sawaragi I, Wynn RM (1969) Ultrastructural localization of metabolic enzymes during the human endometrial cycle. Obstet Gynecol 34:50–61Google Scholar
  34. Schmidt-Matthiesen H (1968) Endometrium und Nidation beim Menschen. Z Geburtsh Gynäkol 168:113–125Google Scholar
  35. Wattenberg LW, Leong JL (1960) Effect of Coenzyme Q10 and menadione on succinic dehydrogenase activity as measured by tetrazolium salt reduction. J Histochem Cytochem 8:296–303Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • E. A. Elias
    • 1
  • R. A. Elias
    • 2
  • A. M. Kooistra
    • 1
  • A. C. Roukema
    • 3
  • J. F. Blacquiere
    • 3
  • H. Barrowclough
    • 4
  • A. E. F. H. Meijer
    • 4
  1. 1.Central Laboratory for National HealthLeeuwardenThe Netherlands
  2. 2.St. Lucas HospitalWinschotenThe Netherlands
  3. 3.De Tjongerschans HospitalHeerenveenThe Netherlands
  4. 4.Department of PathologyAcademic Hospital Wilhelmina GasthuisAmsterdamThe Netherlands

Personalised recommendations