Summary
The effects of papaverine upon force of contraction, maximal rate of contraction, maximal rate of relaxation and 45Ca efflux were studied in isolated superfused rat left atria electrically driven at 1 Hz.
Papaverine (3×10−5 mol/l, increased developed tension (from 5.35±1.17 mN to 7.18±1.51 mN) by 1.8±0.41 mN (+33%, p<0.01) and maximal rate of contraction (+T) by 34.5±13% (p<0.05).
In all experimental conditions tested, papaverine increased the rate of 45Ca efflux. The amount by which papaverine increased 45Ca efflux was 175±41 nmoles · g wet wt−1 and 304±76 nmoles · g wet wt−1, in the presence and in the absence of caffeine, respectively.
The plot of changes in 45Ca efflux versus changes in developed tension fitted to a straight line (r=+0.56, n=17, p<0.05), with a slope and intercept of 42 nmoles Ca · mN−1 · g wet wt−1 and −0.47 mN respectively, suggesting an association between the changes induced by papaverine in force development and increased 45Ca efflux.
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References
Aramendia P, Márquez MT (1982) Papaverine induced changes on cardiac inotropism with special reference to a D-propranolol antagonism. Methods Find Exp Clin Pharmacol 4:229–236
Beavo JA, Rogers NL, Crofford OB, Hardman JG, Sutherland EW, Newman EV (1970) Effects of xanthine derivatives on lipolysis and adenosine 3′,5′-monophosphate phosphodiesterase. Mol Pharmacol 6:597–603
Bers DM, Philipson KD, Langer GA (1981) Cardiac contractility and sarcolemmal calcium binding in several cardiac muscle preparations. Am J Physiol 240 (Heart Circ Physiol 9): H576-H583
Blinks JR, Olson CB, Jewel BR, Braveny P (1972) Influences of caffeine and other methylxanthines on mechanical properties of isolated mammalian hearth muscle. Circ Res 30:367–392
Documenta Geigy (1962) Scientific tables. 6th edn. Dien K (ed), New York, p 178
Fabiato A, Fabiato F (1979) Calcium and cardiac excitation —contraction coupling. Annu Rev Physiol 41:473–484
Holzmann S, Meinertz T, Nawrath H, Scholz H (1977) Effects of papaverine on cyclic AMP, calcium uptake and force of contraction in isolated guinea-pig auricles. Res Commun Chem Phathol Pharmacol 16:443–450
Korth M (1978) Effects of several phosphodiesterase-inhibitors on guinea-pig myocardium. Naunyn-Schmiedeberg's Arch Pharmacol 302:77–86
Márquez MT, Ponce-Hornos JE, Aramendía P (1984) Simultaneous analyses of Ca exchange and mechanical parameters in the rat atria. Methods Find Exp Clin Pharmacol 6:755–761
Nawrath H (1976) Cyclic AMP and cyclic GMP may play opposing roles in influencing force of contraction in mammalian myocardium. Nature 262:509–511
Nawrath H (1980) Papaverine decreases the efflux of 45K in guinea-pig atrial heart muscle. Naunyn-Schmiedeberg's Arch Pharmacol 312:183–185
Nawrath H (1981) Action potential, membrane currents and force of contraction in cat ventricular heart muscle treated with papaverine. J Pharmacol Exp Ther 218:544–549
Nawrath H, Meinertz T (1977) Electrical and mechanical activity of mammalian heart muscle fibres treated with papaverine. Interaction with isoprenaline and dibutyryl cyclic AMP. Naunyn-Schmiedeberg's Arch Pharmacol 299:253–258
Pöch G, Kukovetz WR (1971) Papaverine-induced inhibition of phosphodiesterase activity in various mammalian tissues. Life Sci 10:133–134
Reinhardt D, Roggenbach W, Brodde O-E, Schümann HJ (1977) Influence of papaverine and isoprenaline on contractility and cyclic AMP level of left guinea-pig atria at differents rates of beat. Naunyn-Schmiedeberg's Arch Pharmacol 299:9–15
Reuter H, Scholz H (1977) The regulation of the calcium conductance of cardiac muscle by adrenaline. J Physiol (Lond) 264:49–62
Ringer SA (1883) A further contribution regarding the influence of the different constituents of the blood on the contraction of the heart. J Physiol (Lond) 4:29–42
Sanguinetti MC, West TC (1981) Effects of papaverine on Ca2+-dependent action potentials in guinea-pig myocardium depolarized by potassium. J Pharmacol Exp Ther 219:715–722
Schneider JA, Brooker G, Sperelakis N (1975) Papaverine blockade of an inward slow Ca2+ current in guinea-pig heart. J Mol Cell Cardiol 7:867–876
Shine K, Langer G (1971) Caffeine effects upon contraction and calcium exchange in rabbit myocardium. J Mol Cell Cardiol 3:255–270
Shine MI, Serena SD, Langer GA (1971) Kinetic localization of contractile calcium in rabbit myocardium. Am J Physiol 221:1408–1417
Vassort G, Rougier O, Garnier D, Sauviat MP, Coraboeuf E, Gargouil YM (1969) Effects of adrenaline on membrane inward currents during the cardiac action potential. Pflügers Arch 309:70–81
Weber A, Herz R (1968) The relationship between caffeine contracture of intact musle and the effect of caffeine on reticulum. J Gen Physiol 52:750–759
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Márquez, M.T., Ponce-Hornos, J.E. & Aramendía, P. Effects of papaverine on calcium efflux and contractility in superfused rat left atria. Naunyn-Schmiedeberg's Arch. Pharmacol. 333, 168–171 (1986). https://doi.org/10.1007/BF00506521
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DOI: https://doi.org/10.1007/BF00506521