Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 292, Issue 1, pp 15–20 | Cite as

Peptide and acetylcholine action on neurones of the cat subfornical organ

  • Dominik Felix


Angiotensin II, related oligopeptides and acetylcholine were tested on neurones of the cat subfornical organ (SFO). Angiotensin II activates SFO-neurones by local administration onto the surface, by i.v. injection or with the aid of microiontophoretic techniques. Application of related oligopeptides, bradykinin, physalaemin and eledoisin showed no comparable results. Activation of neurones similar to those observed after angiotensin II was obtained with acetylcholine. About 30% of the cells tested were excited by both substances, 56% of tested SFO-cells responded only to angiotensin II, but not to acetylcholine. Atropine sulphate prevents specifically the acetylcholine excitation.

Since angiotensin II is involved in regulatory mechanism of thirst, these results suggest the possibility that the SFO is one of the sites, where dipsogenic receptors for this circulating peptide are located.

Key words

Subfornical organ Angiotensin II Oligopeptides Acetylcholine Thirst mechanism 


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  1. Akert, K.: Subfornical organ and cholinergic activity. In: Zirkumventrikuläre Organe und Liquor, G. Sterba, ed., pp. 89–93. Symposium in Schloß Reinhardsbrunn. Jena: G. Fischer 1969Google Scholar
  2. Akert, K., Steiner, F. A.: The ganglion psalterii (Spiegel). A brief review of anatomical and physiological aspects of the subfornical organ in mammals. Top. Psychiat. Neurol. 10, 1–14 (1970)Google Scholar
  3. Andersson, B.: Thirst and brain control of water balance. Amer. Scientist. 59, 408–415 (1971)Google Scholar
  4. Andersson, B., Eriksson, L., Oltner, R.: Further evidence for angiotensin-sodium interaction in central control of fluid balance. Life Sci. 9, 1091–1096 (1970)Google Scholar
  5. Andersson, B., Westbye, O.: Synergistic action of sodium and angiotensin on brain mechanisms controlling fluid balance. Life Sci. 9, 601–608 (1970)Google Scholar
  6. Biscoe, T. J., Straughan, D. W.: Microelectrophoretic studies of neurons in cat hippocampus. J. Physiol. (Lond.) 183, 341–359 (1966)Google Scholar
  7. Blass, E. M.: Evidence for basal forebrain thirst osmoreceptors in rat. Brain Res. 82, 69–76 (1974)Google Scholar
  8. Buggy, J., Fisher, A. E., Hoffman, W. E., Johnson, A. K., Phillips, M. I.: Ventricular obstruction: Effect on drinking induced by intracranial angiotensin. Science 190, 72–74 (1975)Google Scholar
  9. Epstein, A. N., Fitzsimons, J. T., Rolls, B. J.: Drinking induced by injection of angiotensin II into the brain of the rat. J. Physiol. (Lond.) 210, 457–474 (1970)Google Scholar
  10. Felix, D.: Angiotensin II in the subfornical organ (SFO). Experientia (Basel) 30, 678 (1974)Google Scholar
  11. Felix, D., Akert, K.: The effect of angiotensin II on neurones of the cat subfornical organ. Brain Res. 76, 350–353 (1974)Google Scholar
  12. Fischer-Ferraro, C., Nahmod, V. E., Goldstein, D. J., Finkielman, S.: Angiotensin and renin in rat and dog brain. J. exp. Med. 133, 353–361 (1971)Google Scholar
  13. Fitzsimons, J. T.: Thirst. Physiol. Rev. 52, 468–561 (1972)Google Scholar
  14. Fitzsimons, J. T.: Angiotensin as a thirst regulating hormone. In: Endocrinology, R. O. Scow, F. J. G. Ebling, and I. W. Henderson, eds., pp. 711–716. Proc. IV. Int. Congress of Endocrinology, Washington, D. C., 1972. Amsterdam: Excerpta Medica, 1973Google Scholar
  15. Ganten, D., Marquez-Julio, A., Granger, P., Hayduk, K., Karsunky, K. P., Boucher, R., Genest, J.: Renin in dog brain. Amer. J. Physiol. 221, 1733–1737 (1971)Google Scholar
  16. Lewis, P. R., Shute, C. C. D.: The cholinergic limbic system: projections to hippocampal formation, medial-cortex, nuclei of the ascending cholinergic reticular system and the subfornical organ and supra-optic crest. Brain 90, 521–540 (1967)Google Scholar
  17. Mulrow, P. J., Goffinet, J. A.: The renin-angiotensin system. In: Physiology of the human kidney, L. G. Wesson, ed., pp. 465–520. New York: Grune and Stratton 1969Google Scholar
  18. Myers, R. D., Hall, G. H., Rudy, T. A.: Drinking in the monkey evoked by nicotine or angiotensin II microinjected in hypothalamic and mesencephalic sites. Pharmacol. Biochem. and Behaviour 1, 15–22 (1973)Google Scholar
  19. Nicoll, R. A., Barker, J. L.: Excitation of supraoptic neurosecretory cells by angiotensin II. Nature New Biol. 223, 172–174 (1971)Google Scholar
  20. Page, I. H., Bumpus, F. M.: Angiotensin. Physiol. Rev. 41, 331–390 (1961)Google Scholar
  21. Panisset, J. C.: Effect of angiotensin on the release of acetylcholine from preganglionic and postganglionic nerve endings. Canad. J. Physiol. Pharmacol. 45, 313–317 (1967)Google Scholar
  22. Peart, W. S.: The renin-angiotensin system. Pharmacol. Rev. 17, 143–182 (1965)Google Scholar
  23. Pfenninger, K., Akert, K., Sandri, C., Bruppacher, H.: Zum Feinbau des Subfornikalorgans der Katze. III. Nerven- und Gliazellen. Schweiz. Arch. Neurol. Neurochir. Psychiat. 100, 232–254 (1967)Google Scholar
  24. Phillips, M. I., Leavitt, M., Hoffman, W.: Experiments on angiotensin II and the subfornical organ in the control of thirst. Fed. Proc. 33, 563 (1974)Google Scholar
  25. Severs, W. B., Daniels-Severs, A. E.: Effects of angiotensin on the central nervous system. Pharmacol. Rev. 25, 415–449 (1973)Google Scholar
  26. Severs, W. B., Daniels-Severs, A., Summy-Long, J., Radio, G. J.: Effects of centrally administered angiotensin II on salt and water excretion. Pharmacology 6, 242–252 (1971)Google Scholar
  27. Shute, C. C. D., Lewis, P. R.: Cholinergic and monoaminergic pathways in the hypothalamus. Brit. med. Bull. 22, 221–226 (1966)Google Scholar
  28. Simpson, J. B., Routtenberg, A.: Subfornical organ: site of drinking elicitation by angiotensin II. Science 181, 1172–1175 (1973)Google Scholar
  29. Simpson, J. B., Routtenberg, A.: Subfornical organ: acetylcholine application elicits drinking. Brain Res. 79, 157–164 (1974)Google Scholar
  30. Simpson, J. B., Routtenberg, A.: Subfornical organ lesions reduce intravenous angiotensin-induced drinking. Brain Res. 88, 154–161 (1975)Google Scholar
  31. Steiner, F. A.: Influence of microelectrophoretically applied acetylcholine on the responsiveness of hippocampal and lateral geniculate neurones. Pflügers Arch. 303, 173–180 (1968)Google Scholar
  32. Yang, H. Y. T., Neff, N. H.: Distribution and properties of angiotensin converting enzyme of rat brain. J. Neurochem. 19, 2443–2450 (1972)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Dominik Felix
    • 1
  1. 1.Hirnforschungsinstitut der Universität ZürichZürichSwitzerland

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