Skip to main content
Log in

The localization of enzyme activities in the pancreatic appendages of Sepia officinalis L. (Cephalopoda)

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

In this study, enzyme activities of the pancreatic appendages of the ductus hepatopancreas (the so-called “Pancreas”) in Sepia officinalis L. have been demonstrated by light and electron microscopical methods: Malate dehydrogenase, monoamine oxidase, acid phosphatase, β-glucuronidase, adenosine triphosphatase and carbonic anhydrase were shown by the former, and monoamine oxidase, catalase, glutamic oxalacetic transaminase, choline esterase (non-specific), alkaline phosphatase, acid phosphatase and carbonic anhydrase by the latter technique.

The correlation between enzyme activity and distribution, and the presumed function of the two pancreatic epithelia is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bidder, A.M.: Feeding and digestion in cephalopods. In: Physiology of Mollusca. Wilbur, K.M., Yonge, C.M., (eds.). New York, London: Academic Press 1966

    Google Scholar 

  • Boadle, M.C., Bloom, F.E.: A method for the structural demonstration of monoamine oxidase activity. III. Internat. Congr. Histochem. Cytochem. New York 1968

  • Boucaud-Camou, E.: Etude histologique et histochimique de l'appareil digestif de Sepoila atlantica D'Orbigny et Sepia officinalis L. Bull Soc. Linné Normandie 9, 220–243 (1968)

    Google Scholar 

  • Boucaud-Camou, E.: Etude infrastructurale du pancréas de Sepia officinalis L. Bull. Soc. Zool. France 97, 197–203 (1972)

    Google Scholar 

  • Boucaud-Camou, E.: Localisation d'activités enzymatiques impliquées dans la digestion chez Sepia officinalis L. Arch. Zool. Exp. Gén. 115, 5–27 (1974)

    Google Scholar 

  • Buchwalow, I.B., Unger, E., Schulze, W., Schön, R., Raikhlin, N.T.: Hemmung und Stimulierung der ATPase-Aktivität und die nichtenzymatische Hydrolyse von ATP in der elektronenmikroskopischen Histochemie. Histochemistry 44, 1–11 (1975)

    Google Scholar 

  • Burck, H.C.: Histologische Technik. Stuttgart: Thieme 1969

    Google Scholar 

  • DeDuve, C.: Function of microbodies (Peroxisomes). J. Cell Biol. 27, 25A (1965)

    Google Scholar 

  • von Deimling, O.: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Stimultan-Methoden. Histochemie 4, 48–55 (1964)

    Google Scholar 

  • Ericcson, J.L.E., Trump, B.F.: Observations on the application of electron microscopy of the lead phosphate technique for the demonstration of acid phosphatase. Histochemie 4, 470–487 (1965)

    Google Scholar 

  • Glenner, G.G., Burtner, H.J., Brown, G.W.: The histochemical demonstration of monoamine oxidase activity by tetrazolium salts. J. Histochem. Cytochem. 5, 591–600 (1957)

    Google Scholar 

  • Goebel, A., Puchtler, H.: Zur Darstellung der Carboanhydrase im histologischen Schnitt. Naturwissenschaften 41, 531–532 (1954b)

    Google Scholar 

  • Hansson, H.P.J.: Histochemical demonstration of carbonic anhydrase activity. Histochemie 11, 112–128 (1967)

    Google Scholar 

  • Jokota, S.: Electron microscopic demonstration of carbonic anhydrase activity in mouse liver cells. Histochemie 19, 255–261 (1969)

    Google Scholar 

  • Karnovsky, M.J., Himmelhoch, S.R.: Histochemical localization of glutaminase I activity in kidney. Am. J. Physiol. 201, 786–790 (1961)

    Google Scholar 

  • Kasa, P., Csillik, B.: Electron microscopic localization of choline esterase by a copper-lead-thiocholine technique. J. Neurochem. 13, 1345–1349 (1966)

    Google Scholar 

  • Lee, S.H.: The possible role of the vesicles in renal ammonia excretion. An implication of concentrated glutamic oxalacetic transaminase. J. Cell. Biol. 45, 644–649 (1970)

    Google Scholar 

  • Legg, P.G., Wood, R.L.: Effects of catalase inhibitors on the ultrastructure and peroxidase activity of proliferating microbodies. Histochemie 22, 262–276 (1970)

    Google Scholar 

  • Lolova, I., Dikov, A.: Histochemical evidence of aminotransferase. IV. Histochemical and electrophoretical investigation of aminotranferases in rat organs. Acta Histochem. (Jena) 53, 12–27 (1975)

    Google Scholar 

  • Mayahara, H., Hirano, H., Saita, T., Ogawa, K.: The new lead citrate method for the ultracytochemical demonstration of activity of nonspecific alkaline phosphatase (orthophosphoric monoester phosphohydrolase). Histochemie 11, 88–96 (1967)

    Google Scholar 

  • Müller, J., DaLage, C.: Ultracytochemical demonstration of monoamine oxidase activity in nervous and non-nervous tissues of the rat. J. Histochem. Cytochem. 25, 337–348 (1977)

    Google Scholar 

  • Panula, P., Rechardt, L.: Age-dependent increase in the non-specific cholinesterase activity of the capillaries in the rat neostriatum. Histochemistry 55, 49–54 (1978)

    Google Scholar 

  • Pearse, A.G.E.: Histochemistry. Theoretical and applied. 2nd and 3rd eds. London: Churchill 1961/1968

    Google Scholar 

  • Potts, W.T.W.: Ammonia excretion in Octopus dofleini. Comp. Biochem. Physiol. 14, 339–355 (1965)

    Google Scholar 

  • Romijn, C.: Die Verdauungsenzyme bei einigen Cephalopoden. Arch. Neerl. Zool. 1, 373–431 (1935)

    Google Scholar 

  • Sasse, D.: Glykogen in der Ontogenese des Verdauungstraktes-chemomorphologische und stoff-wechselhistochemische Analyse. Ergebn. Anat. Entwickl.-Gesch. 40/2, 1–68 (1968)

    Google Scholar 

  • Schäfer, A., Höhn, P., Mika, H., Allbach, G.: Enzymhistochemische und elektronenmikroskopische Untersuchungen an der Darmschleimhaut der Ratte im Tagesrhythmus. Acta Histochem. (Jena) 48, 301–319 (1974)

    Google Scholar 

  • Schipp, R., Höhn, P., Schäfer, A.: Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhangs (Pericardialdrüse) von Sepia officinalis. Z. Zellforsch. 117, 252–274 (1971)

    Google Scholar 

  • Schipp, R., v. Boletzky, S., Doell, G.: Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusca, Cephalopoda). Z. Morphol. Tiere 81, 279–304 (1975)

    Google Scholar 

  • Schipp, R., v. Boletzky, S.: The pancreatic appendages of dibranchiate cephalopods. I. The fine structure of the “pancreas” in Sepioidea. Zoomorphologie 86, 81–98 (1976)

    Google Scholar 

  • Schipp, R.: An indirect demonstration of the substructure of the lamina basalis in the branchial heart of Sepia officinalis L. by means of cholinesterase reaction. Experientia 33, 74 (1977)

    Google Scholar 

  • Seligman, A.M., Tsou, K.C., Rutenburg, S.H., Cohen, R.B.: Histochemical demonstration of β-glucuronidase with a synthetic substrate. J. Histochem. Cytochem. 2, 209–229 (1954)

    Google Scholar 

  • Shannon, W.A., Hannah, J., Wasserkrug, L., Seligman, A.M.: The ultrastructural localization of monoamine oxidase (MAO) with tryptamine and a new tetrazolium salt. 2-(2′-benzothiazolyl)-5-styryl-3-(4′-phthalhydrazidyl) tetrazolium chloride. J. Histochem. Cytochem. 22, 170–182 (1974)

    Google Scholar 

  • Stockinger, L.: Fermentnachweise im Ultrastrukturbereich. In: Histochemie der Ultrastruktur. Acta Histochem. (Jena) Suppl. X, 27–63 (1971)

  • Veenhuis, M., Bonga, S.D.W.: The cytochemical demonstration of catalase and D-amino acid oxidase in the microbodies of teleost kidney cells. Histochem. J. 9, 171–181 (1977)

    Google Scholar 

  • Williams, G., Jackson, D.S.: Two organic fixatives for acid mucopolysaccharids. Stain Technol. 31, 189–191 (1956)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This study was supported by the “Deutsche Forschungsgemeinschaft”

Rights and permissions

Reprints and permissions

About this article

Cite this article

Donaubauer, H.H., Schipp, R. The localization of enzyme activities in the pancreatic appendages of Sepia officinalis L. (Cephalopoda). Histochemistry 59, 29–44 (1978). https://doi.org/10.1007/BF00506475

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00506475

Keywords

Navigation