Summary
Spontaneous activity of the isolated cardiac pacemaker, investigated by means of microelectrodes, ceases under the influence of reserpine or α-methyl p-tyrosine. It may be restored either by washing the preparation with Tyrode solution or by adding catecholamines to the perfusing fluid containing reserpine or α-methyl p-tyrosine. In the majority of experiments it was also possible to restore the spontaneous activity with dibutyryl cyclic AMP added to the perfusing fluid containing one of the two inhibitors mentioned above.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahren, K., Hjalmarson, A., Isacson, O.: Inotropic and metabolic effects of dibutyryl adenosine 3′5′monophosphate in the perfused rat heart. Acta physiol. scand. 82, 79–90 (1971).
Angelakos, E. T.: Regional distribution of catecholamines in dog heart. Circulat. Res. 16, 39–44 (1965)
Angelakos, E. T., Fuxe, K., Torchiana, M. L.: Chemical and histochemical evaluation of distribution of catecholamines in the rabbit and guinea pig hearts. Acta physiol. scand. 59, 184–192 (1963)
Angelakos, E. T., King, M. P., Millard, R. W.: Regional distribution of catecholamines in the hearts of several species. An. N. Y. Acad. Sci. 156, 219–240 (1969)
Butcher, R. W., Robison, R. A., Sutherland, E. W.: The role of cyclic AMP in certain biological control systems. In: Control processes in multicellular organisms, p. 64. Eds.: E. W. Wolstenholme and J. Knight, London: Churchill 1970.
Dahlström, A., Fuxe, K., Mya-tu, M., Zetterström, B. E.: Observations on adrenergic innervation of dog heart. Amer. J. Physiol. 209, 689–692 (1965)
Elharrar, V., de Champlain, J., Nadeau, R. A.: Effects of 6-hydroxydopamine on the canine sinus node. Circulat. Res. 28, 188–198 (1971)
Euler, U. S.: Effect of some metabolic factors and drugs on uptake and release of catecholamines in vitro and in vivo. In: New aspects of storage and release mechanisms of catecholamines. Bayer Symposium II, p. 144. Eds.: H. J. Schümann and G. Kroneberg. Berlin-Heidelberg-New York: Springer 1970
Friedman, W. F., Pool, P. E., Jacobowitz, D., Seagren, S. C., Braunwald, E.: Sympathetic innervation of the developing rabbit heart. Circulat. Res. 23, 25–32 (1968)
Gartner, S. L., Vahouny, G. V.: Effects of epinephrine and cyclic 3′5′-AMP on perfused rat hearts. Amer. J. Physiol. 222, 1121–1124 (1972)
Jacobowitz, D.: Histochemical studies of the relationship of chromaffin cells and adrenergic nerve fibres to cardiac ganglia of several species. J. Pharmacol. exp. Ther. 158, 227–240 (1967)
Jamieson, J. D., Palade, G. E.: Specific granules in atrial muscle cells. J. Cell Biol. 23, 151–172 (1964)
Krause, E. G., Halle, W., Kallabis, E., Wollenberger, A.: Positive chronotropic response of cultured isolated rat heart cells to N6, 2′-O-dibutyryl 3′5′-adenosine monophosphate. J. molec. cell. Cardiol. 1, 1–10 (1970)
Kokovetz, W. R.: Über die Wirkung von Dibutyryl-3′5′-AMP am isolierten Herzen. Naunyn-Schmiedeberg's Arch. Pharmak. exp. Path. 260, 163–164 (1968)
Kukovetz, W. R., Pöch, G.: Cardiostimulatory effects of cyclic-3′5′-adenosine monophosphate and its acylated derivatives. Naunyn-Schmiedegergs Arch. Pharmak. 266, 236–254 (1970)
Lu, H. H., Lange, G., Brooks, Ch. McC.: Factors controlling pacemaker actions in cells of the sinus node. Circulat. Res. 17, 460–471 (1965)
Meinertz, T., Nawrath, yH., Scholz, H.: Über die positiv inotrope Wirkung von Dibutyryl-3′5′-AMP an isolierten Rattenvorhöfen. Pflügers Arch. 333, 197–212 (1972)
Paasonen, M. K., Krayer, O.: The release of norepinephrine from mammalian heart by reserpine. J. Pharmacol. exp. Ther. 123, 153–160 (1958)
Palade, G. E.: Secretory granules in the atrial myocardium. Anat. Rec. 139, 262 (1961)
Potter, L. T., Cooper, T., Willman, V. L., Wolfe, D. E.: Synthesis, binding, release and metabolism of normal and transplanted dog hearts. Circulat. Res. 16, 468–481 (1965)
Rutledge, C. O., Weiner, N.: The effect of reserpine upon the synthesis of norepinephrine in the isolated rabbit heart. J. Pharmacol. exp. Ther. 157, 290–302 (1967)
Shindler, R., Harakal, C., Sevy, R. W.: Catecholamines content of the sinoatrial node and common right atrial tissue. Proc. Soc. exp. Biol. (N.Y.) 128, 798–800 (1968)
Skelton, C. L., Levey, G. S., Epstein, S. E.: Positive inotropic effects of dibutyryl cyclic adenosine 3′5′-monophosphate. Circulat. Res. 26, 35–43 (1970)
Smith, A. D.: Subcellular localisation of noradrenaline in sympathetic neurons. Pharmacol. Rev. 24, 435–457 (1972)
Sosa-Lucero, J. C., DeLa Iglesia, F. A., Lumb, G., Berger, J. M., Bencosme, S.: Subcellular distribution of catecholamines and specific granules in rat heart. Lab. Invest. 21, 19–26 (1969)
Spector, S., Sjoerdsma, A., Udenfriend, S.: Blockade of endogenous norepinephrine synthesis by α-methyl-tyrosine, and inhibitor of tyrosine hydroxylase. J. Pharmacol. exp. Ther. 147, 86–95 (1965)
Sutherland, E. W., Robinson, G. A., Butcher, R. W.: Some aspects of the biological role of adenosine 3′5′-monophosphate (Cyclic AMP). Circulation 37, 279–306 (1968)
Toda, N.: Effects of adrenaline, noradrenaline and reserpine on the transmembrane potential in both pacemaker and non-pacemaker fibres of the rabbit atrium. Jap. J. Pharmacol. 10, 78–91 (1960)
Tuganowski, W.: Reactivation of bioelectrical activity of the pacemaker of dying mammalian heart. Acta physiol. pol. 17, 901–914 (1966)
Tuganowski, W., Wolański, A.: Effect of α-methyl Dopa on the spontaneous activity of the pacemaker of the heart. Acta physiol. pol. 19, 805–811 (1968)
Udenfriend, S., Zaltzman-Nirenberg, P., Nagatsu, T.: Inhibitors of purified beef adrenal tyrosine hydroxylase. Biochem. Pharmacol. 14, 837–846 (1965)
Weiner, N., Rutledge, C. O.: The actions of reserpine on the biosynthesis and storage of catecholamines. In: Mechanism of release of biogenic amines, p. 307. Eds.: U. S. von Euler, S. Rosell, and B. Uvnäs. New York: Pergamon Press 1966
Author information
Authors and Affiliations
Additional information
This work was supported by the Polish Academy of Science (Grant No. 251/VI).
Rights and permissions
About this article
Cite this article
Tuganowski, W., Krause, M. & Korczak, K. The effect of dibutyryl 3′5′-cyclic AMP on the cardiac pacemaker, arrested with reserpine and α-methyl-tyrosine. Naunyn-Schmiedeberg's Arch. Pharmacol. 280, 63–70 (1973). https://doi.org/10.1007/BF00505355
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00505355