Uranium resistance of Thiobacillus ferrooxidans
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The toxicity of uranium to several isolates of Thiobacillusferrooxidans was determined. Plasmid DNA of approx. 13±0.5 megadaltons was detected in four cultures that exhibited the highest resistance to UO22+. One isolate, TFI-7, always contained a 13 megadalton plasmid and was always resistant to uranium. Another isolate, TFI-13, showed increased resistance to uranium only after a long lag period during which the cell numbers declined 1000-fold when uranium was added to the medium. The surviving TFI-13 population was able to grow with no further loss of viability upon re-exposure to uranium and it contained a 13 megadalton plasmid. Upon subculture in the absence of uranium this resistance declined. Concurrent with the loss of uranium resistance, the 13 megadalton plasmid could no longer be detected. Attempts to cure plasmids by elevated temperatures and chemical agents were unsuccessful.
KeywordsToxicity Uranium Elevated Temperature High Resistance Chemical Agent
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- Derry R, Garrett KH, Le Roux NW, Smith SE (1976) Bacterially assisted plant process for leaching uranium ores. In: Jones MJ (ed) Geology, mining and extractive processing of uranium. Institution of Mining and Metallurgy, London, pp 56–62Google Scholar
- DiSpirito AA, Silver M, Voss L, Tuovinen OH (1982) Flagella and pili of iron-oxidizing thiobacilli isolated from a uranium mine in northern Ontario, Canada. Appl Environ Microbiol 43:1196–1200Google Scholar
- Duncan DW, Bruynesteyn A (1971) Enhancing bacterial activity in a uranium mine. Can Min Metall Bull 64 (709):32–36Google Scholar
- Guay R, Silver M, Torma AE (1977) Ferrous iron oxidation and uranium extraction by Thiobacillus ferrooxidans. Biotechnol Bioengin 19:727–740Google Scholar
- Harrison VF, Gow WA, Ivarson KC (1966) Leaching of uranium from Elliot Lake ore in the presence of bacteria. Can Min J 87 (5):64–67Google Scholar
- Martin PAW, Dugan PR, Tuovinen OH (1981) Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Can J Microbiol 27:850–853Google Scholar
- Tomizuka N, Takahara Y (1972) Bacterial leaching of uranium from Ningyo-toge ores. In: Terui G (ed) Fermentation technology today. Society for Fermentation Technology, Osaka, pp 513–520Google Scholar
- Tuovinen OH, Kelly DP (1974) Studies on the growth of Thiobacillus ferrooxidans. II. Toxicity of uranium to growing cultures and tolerance conferred by mutation, other metal cations and EDTA. Arch Microbiol 95:153–164Google Scholar
- Tuovinen OH, Silver M, Martin PAW, Dugan PR (1981) The Agnew Lake uranium mine leach liquors: chemical examinations, bacterial enumeration, and composition of plasmid DNA of iron-oxidizing thiobacilli. In: Proceedings of the international conference on use of microorganisms in hydrometallurgy. Hungarian Academy of Sciences, Pécs, pp 59–69Google Scholar