Microcalorimetry: A tool for the study of the biodegradability of straw by mixed bacterial cultures

  • Marie-Laure Fardeau
  • Françoise Plasse
  • Jean-Pierre Belaich
Industrial Microbiology

Summary

The biodegradability of straw by a mixed bacterial culture obtained from a pile of weeds was studied by microcalorimetry. All the cultures were grown at 30°C under anaerobic conditions in microcalorimetric vessels. The fermentation thermograms, obtained using well defined conditions, were very reproducible. The quantities of heat produced during straw degradation were found to be proportional to the quantity of straw introduced at the beginning of the fermentation.

The recovered carbon was also found to be proportional to the initial quantity of straw. From both microcalorimetric and chemical analysis it was concluded that the limiting factor of the straw degradation was the cellulolytic activity of the mixed culture. This is supported by the fact that commercially available cellulase added to the growth medium increases the amount of straw degradation by about four times. The heat associated with fermentation of each cellulose monomer (C6H10O5) was found to be 120 kJ, a value which is close to the heat associated with hexose fermentation by pure cultures. In conclusion, we propose that microcalorimetry can be used as a powerful tool for the analysis of the biodegradability of complex heterogeneous substrate by pure or mixed cultures.

Keywords

Cellulose Chemical Analysis Fermentation Cellulase Straw 

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References

  1. Battley EH (1960) Enthalpy changes accompanying the growth of Saccharomyces cerevisiae. Physiol Plant 13: 628–639Google Scholar
  2. Belaich A, Belaich JP 1976a Microcalorimetric study of the anaerobic growth of Escherichia coli: growth thermograms in a synthetic medium. J Bacteriol 125: 14–18Google Scholar
  3. Belaich A, Belaich JP 1976b Microcalorimetric study of the anaerobic growth of Escherichia coli: Measurement of the affinity of whole cells for various energy substrates. J Bacteriol 125: 19–24Google Scholar
  4. Belaich JP, Senez JC, Murgier M (1968) Mircrocalorimetric study of glucose permeation in microbial cells. J Bacteriol 95: 1750–1757Google Scholar
  5. Bryant MP, Tzeng SF, Robinson IM, Joyner AE (1970) Nutrient requirements of methanogenic bacteria. Adv Chem Ser 105: 23–40Google Scholar
  6. Clausen EC, Sitton OC, Gaddy JL (1977) Bioconversion of crop materials to methane. Process Biochem 12: 5–7Google Scholar
  7. Diaz LF, Trezek GJ (1977) Biogasification of a selected fraction of municipal solid wastes. Compost Sci 18: 8–13Google Scholar
  8. Forrest WW (1969) Enthalpy changes associated with the lactic fermentation of glucose. In: Brown HD (ed) Biochemical microcalorimetry. Academic Press, New York, London, pp 165–180Google Scholar
  9. Fry LJ (1974) Practical Building of methane power plants In:Knox DA (ed) LJ Fry, Santa Barbara, CaliforniaGoogle Scholar
  10. Golueke CG (1976) Composting: a review of rationale, principles and public health. Compost Sci 17: 11–15Google Scholar
  11. Gosz JR, Homes RT, Likens GE, Bormann FH (1978) The flow of energy in a forest ecosystem. Sci Am 237: 93–102Google Scholar
  12. Hall DO (1977) Solar energy and biology for fuel, food and fibre TIBS, vol 5: 99–101Google Scholar
  13. Hart SA (1963) Digestion tests of livestock wastes. Water Pollut Control Fed J 35: 748–757Google Scholar
  14. Hawkes D, Horton R, Stafford DA (1976) The application of anaerobic digestion to producing methane gas and fertilizer from farm wastes. Process Biochem 12: 32–35Google Scholar
  15. Hobson PN, Shaw BG (1973) The anaerobic digestion of waste from an intensive pig unit. Water Research. Pergamon Press, New York, 7: 437–448Google Scholar
  16. Jewell WJ, Loehr RC (1977) In: Taiganides EP (ed) Animal wastes. Applied Sciences Publishers LTD, London pp 241–250Google Scholar
  17. Klass DL, Ghosh S (1973) Fuel gas from organic wastes. Chem Technol 3: 689–698Google Scholar
  18. Mah RA, Ward DM, Baresi L, Glass PL (1977) Biogenesis of methane. Annu Rev Microbiol 31: 309–341Google Scholar
  19. Murgier M, Belaich JP (1971) Microcalorimetric determination of the affinity of Saccharomyces cerevisiae for some carbohydrate growth substrates. J Bacteriol 105: 573–579Google Scholar
  20. Octagon Papers n° 3 (1976) Cellulosic substrates. Powell AJ, Bu'Lock JD (eds). Department of Extra-Mural Studies, The University ManchesterGoogle Scholar
  21. Parikh JK, Parikh KS (1976) Seminar Göttingen: Schlegel HG (ed) Microbial energy conversion. Pergamon Press, New York, pp 555–591Google Scholar
  22. Parrard P (1956) La production de méthane biologique conduite en toute saison. Agriculture London 179: 134–139Google Scholar
  23. Pfeffer JT (1978) Methane from urban solid wastes. Process Biochem 13: 8–11Google Scholar
  24. Prevot AR (1977) Maloine SA (ed) Biosynthèse bacteriénne du methane et des pétroles pour l'an 2000.Google Scholar
  25. Savery CW, Cruzon DC (1972) Methane recovery from chicken manure digestion. J Water Pollut Control Fed 44: 2349–2354Google Scholar
  26. Stafford DA (1974) Methane production from waste. Effluent and water treatment J. FEBS 73-79Google Scholar
  27. Varel VH, Isaacson UR, Bryant MP (1977) Thermophilic methane production from cattle waste. Appl Environ Microbiol 33: 298–307Google Scholar
  28. Vaseen DA (1977) New Method of dual media fermentation can produce quality methane. Water Wastes Eng 14: 42–46Google Scholar
  29. Weimer PJ, Zeikus JG (1977) Fermentation of cellulose and cellubiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermoautotrophicum. Appl Environ Microbiol 33: 289–297Google Scholar
  30. Zeikus JG (1977) The biology of methanogenic bacteria. Bacterial Rev 41: 514–541Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Marie-Laure Fardeau
    • 1
  • Françoise Plasse
    • 1
  • Jean-Pierre Belaich
    • 1
  1. 1.Laboratoire de Chimie BactérienneC.N.R.S.Marseille Cedex 2France

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