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Ribonucleotides during the regeneration of the rat kidney after lesions by folic acid, HgCl2 and ligation of the ureter

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Summary

During the regeneration of the rat kidney after lesion by 500 mg folic acid/kg, the ribonucleotide concentrations (ATP, ADP, AMP, UTP, UDP, GTP, GDP) were determined. After 1, 3, and 7 days no significant changes were found, although 1 and 2 h after injection of folic acid a slight decrease in the concentrations of ATP, AMP, GTP, and UTP was measured.

Mitochondria which were isolated from rat kidneys 1 and 4 days after application of folic acid showed an unchanged consumption of O2 and phosphate as compared with the controls. In mitochondria isolated 1 h after folic acid, a sharp decrease in O2-consumption and a complete inhibition of the uptake of inorganic phosphate occurred. However, the incorporation of 32P into ATP under in vivo conditions was not reduced. The unchanged synthesis of energy-rich phosphate compounds explains the rapid onset of the proliferation of the kidney.

24 h after injection of 1.5 mg HgCl2/kg, a fall of approximately 50% in the ATP-concentration was found. In the regenerative phase, only a slight increase in protein content of the kidneys was observed.

Ligation of the ureter did not cause a significant drop of energy-rich adenine nucleotides. During the regenerative phase a pronounced increase in the protein content of the kidney was found.

The injection of folic acid dissolved in 1 M bicarbonate produced no disturbance of kidney function. No increase of dry weight or of protein content resulted. The accumulation and precipitation of folic acid in the kidney tubules are prerequisites for the initiation of the regenerative processes.

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  1. Pharmakologisches Institut der Freien Universität Berlin, Thielallee 68/73, D-1000, Berlin 33

    J. Oertel & H. Herken

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  1. J. Oertel
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  2. H. Herken
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Oertel, J., Herken, H. Ribonucleotides during the regeneration of the rat kidney after lesions by folic acid, HgCl2 and ligation of the ureter. Naunyn-Schmiedeberg's Arch. Pharmacol. 284, 383–393 (1974). https://doi.org/10.1007/BF00504707

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  • DOI: https://doi.org/10.1007/BF00504707

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