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Effects of aeration and of corn steep concentration on L-asparaginase production in Escherichia coli

  • J. Netrval
Industrial Microbiology

Summary

The production of L-asparaginase was investigated in Escherichia coli, growing under different conditions of aeration in a medium containing 2% or 6% corn steep. At both concentrations, excessive aeration decreased enzyme production. In the medium with 2% corn steep, L-asparaginase activity began to decline as soon as the oxygen absorption exceeded 0.22 mmol O2 l−1 min−1, and when the oxygen absorption rate was 1.26 mmol O2 l−1 min−1, enzyme activity reached only about 5% of maximum. In the medium with 6% corn steep, a decline of L-asperaginase activity did not appear until the oxygen absorption rate value exceeded 0.54 mmol O2 l−1 min−1, at the oxygen absorption rate of 1.26 mmol O2 l−1 min−1, the enzyme activity still reached about 50% of maximum.

Keywords

Oxygen Enzyme Escherichia Coli Enzyme Activity Corn 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • J. Netrval
    • 1
  1. 1.Research Institute for Antibiotics and BiotransformationsRoztoky near PragueCSSR

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