Mutational study of the alcohol dehydrogenase-1 FC m duplication in maize
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The alcohol dehydrogenase-1 FC m (Adh-FC m ) duplication in maize was subjected to ethyl methanesulfonate (EMS) mutagenesis. Of the mutants recovered, eight produced ADH polypeptides with altered electrophoretic mobility. Four produced new mobilities of the progenitor F with no change of the Cm molecule; the remainder altered only the Cm enzyme. No cases were found in which the electrophoretic mobilities of the two types of subunits were simultaneously altered, and no complete nulls lacking both F and Cm were recovered. These observations confirm the duplicate nature of the FCm complex.
Key wordsgene duplication alcohol dehydrogenase electrophoretic mobility mutants maize
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