Abstract
Catalytic activity of the atypical Oriental-type aldehyde dehydrogenase-2 (ALDH2) was considered to be null or severely diminished. Recently it was suggested that the atypical ALDH 22 retained about 30% of the specific activity of the usual ALDH 12 . We reexamined the problem by two-dimensional crossed immunoelectrophoresis. The usual Caucasian livers exhibited two distinctive precipitin peaks, one corresponding to the cytosolic ALDH1 and the other corresponding to the usual mitochondrial ALDH 12 , in both protein stain and enzyme activity stain. In contrast, the atypical Oriental livers exhibited two precipitin peaks in protein stain, but only one peak, corresponding to ALDH1, in enzyme activity stain. These results support the original notion that the atypical ALDH 22 is enzymatically inactive or far less active than the usual enzyme, refuting the idea of the atypical ALDH 22 with substantial enzyme activity.
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This work was supported by U.S. Public Health Service Grants HL-29515 and AA05763.
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Yoshida, A., Dave, V. Enzymatic activity of atypical oriental types of aldehyde dehydrogenases. Biochem Genet 23, 585–590 (1985). https://doi.org/10.1007/BF00504292
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DOI: https://doi.org/10.1007/BF00504292