Abstract
Mutations at three second-chromosomal loci of Drosophila melanogaster have been isolated, mapped, and shown to be purine nucleoside auxotrophs. Two of the loci, adenosine2 and adenosine3, located at map positions 18.4 and 20, respectively, produce mutations which are supplementable with adenine, adenosine, and inosine. Guanosine supplements mutations at the burgundy locus (55.7); this locus was described previously through a pteridine eye-color defect but identified as an auxotrophic locus after the isolation of a new allele, bur gua2-1. The mutation ade2-1 also has defective pteridine metabolism.
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Anderson, J. H., and Sartorelli, A. C. (1968). Inosinic acid dehydrogenase of Sarcoma 180 cells. J. Biol. Chem. 2434762.
Beadle, G. W., and Tatum, E. L. (1941). Genetic control of biochemical reactions in Neurospora. Proc. Natl. Acad. Sci. USA 27499.
Becker, J. L. (1974a). Metabolisme des purines dans des cellules de Drosophila melanogaster en culture in vitro: Interconversion des purines. Biochemie 561249.
Becker, J. L. (1974b). Purine metabolism pathways in Drosophila cells grown in vitro: Phosphoribosyltransferase activities. Biochimie 56779.
Boss, G. B., and Seegmiller, J. E. (1982). Genetic defects in human purine and pyrmidine metabolism. Annu. Rev. Genet. 16297.
el Kouni, M. H., and Nash, D. (1974). Biology of 5-fluoro-2′—deoxyuridine sensitivity of Drosophila melanogaster larvae. J. Insect Physiol. 201481.
el Kouni, M. H., and Nash, D. (1977). Survival of Drosophila melanogaster larvae on defined medium supplemented with naturally occurring nucleosides and nucleic acid bases. J. Insect Physiol. 23327.
Falk, D. R., and Nash, D. (1974). Sex-linked auxotrophic and putative auxotrophic mutants of Drosophila melanogaster. Genetics 76755.
Fan, C. L., Hall, L. M., Skrinska, A. J., and Brown, G. M. (1976). Correlation of guanosine triphosphate cyclohydrolase activity and the synthesis of pterins in Drosophila melanogaster. Biochem. Genet. 14271.
Hadorn, E., and Mitchell, H. K. (1951). Properties of mutants of Drosophila melanogaster and changes during development as revealed by paper chromatography. Proc. Natl. Acad. Sci. USA 37650.
Henikoff, S., Tatchell, K., Hall, B. D. and Nasmyth, K. A. (1981). Isolation of a gene from Drosophila by complementation in yeast. Nature 28933.
Jarry, B., and Falk, D. (1974). Functional diversity within the rudimentary locus of Drosophila melanogaster. Mol. Gen. Genet. 135113.
Johnson, M. M. (1978). Cytogenetic and Biochemical Studies on Purine-Requiring Mutants of Drosophila melanogaster, M.Sc. thesis, University of Alberta, Edmonton, Alberta.
Johnson, M. M., Nash, D., and Henderson, J. F. (1977). Biochemical studies of purine auxotrophs of Drosophila melanogaster. In Muller, M. M., Kaiser, E., and Seegmiller, J. E. (eds.), Purine Metabolism in Man. II. Regulation of Pathways and Enzyme Defects Plenum Press, New York, pp. 110–114.
Johnson, M. M., Woloshyn, E. P., and Nash, D. (1979). Cytogenetic localization of the purine1 and guanosine1 loci of Drosophila melanogaster: The purine1 locus specifies a vital function. Mol. Gen. Genet. 174287.
Johnson, M. M., Nash, D., and Henderson, J. F. (1980a). Metabolism of guanine and guanosine in larvae of Drosophila melanogaster. Comp. Biochem. Physiol. 66B563.
Johnson, M. M., Nash, D., and Henderson, J. F. (1980b). Purine metabolism in larvae of Drosophila melanogaster fed radioactive hypoxanthine, inosine or formate. Comp. Biochem. Physiol. 66B555.
Johnstone, M. E., and Nash, D. (1979). IMP dehydrogenase levels in a guanosine-requiring strain of Drosophila melanogaster. Genetics 91s56.
Lastowski, D. M., and Falk, D. R. (1980). Characterization of an autosomal dominant rudimentary shaped wing mutation in Drosophila melanogaster which affects pyrimidine synthesis. Genetics 96471.
Lindsley, D. L., and Grell, E. H. (1968). Genetic Variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. No. 627.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.
MacKay, W. J., and O'Donnell, J. M. (1983). A genetic analysis of the pteridine biosynthetic enzyme, guanosine triphosphate cyclohydrolase, in Drosophila melanogaster. Genetics 10535.
Naguib, F. N. M. (1976). Auxotrophic Mutants of the Second Chromosome of Drosophila melanogaster, Ph.D. thesis, University of Alberta, Edmonton, Alberta.
Naguib, F. N. M., and Nash, D. (1976). Nucleoside auxotrophy in Drosophila: An autosomal locus yielding mutants supplementable by purine and pyrimidine ribonucleosides. Mol. Gen. Genet. 14713.
Nash, D. (1963). Selective Breeding and the Development Control of the Expression of the Hairless Mutant of Drosophila melanogaster, Ph.D. thesis, Cambridge University, Cambridge, England.
Nash, D., and Henderson, J. F. (1982). The biochemistry and genetics of purine metabolism in Drosophila melanogaster. Adv. Comp. Physiol. Biochem. 81.
Nash, D., Woloshyn, E. P., Mehl, Y.-M., and Janca, F. C. (1981). Pleiotropic, recessive lethal mutants associated with purine metabolism in Drosophila melanogaster. Can. J. Genet. Cytol. 23411.
Nolte, D. J. (1954). The eye pigmentary system of Drosophila. V. The pigments of the light and dark groups of mutants. J. Genet. 52127.
Norby, S. (1970). A specific nutritional requirement for pyrimidines in rudimentary mutants of Drosophila melanogaster. Hereditas 66205.
Porter, L. A., and Rawls, J. M. (1984). The Dhod locus of Drosophila: mutations and interrelationships with other loci controlling de novo pyrimidine biosynthesis. Mol. Gen. Genet. 19327.
Puck, T. T., and Kao, F. T. (1982). Somatic cell genetics and its application to medicine. Annu. Rev. Genet. 16225.
Rawls, J. M., Jr. (1980). Identification of a small genetic region that encodes orotate phosphoribosyltransferase and orotidylate decarboxylase in Drosophila melanogaster. Mol. Gen. Genet. 17843.
Rawls, J. M., and Fristrom, J. W. (1975). A complex genetic locus that controls the first three steps of pyrimidine biosynthesis in Drosophila. Nature 255738.
Sang, J. H. (1956). The quantitative nutritional requirements of Drosophila melanogaster. J. Exp. Biol. 3345.
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This work was supported by NSERC Grant A3269 to D. Nash and by an AHFMR graduate studentship to M. E. Johnstone.
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Johnstone, M.E., Nash, D. & Naguib, F.N.M. Three purine auxotrophic loci on the second chromosome of Drosophila melanogaster . Biochem Genet 23, 539–555 (1985). https://doi.org/10.1007/BF00504289
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DOI: https://doi.org/10.1007/BF00504289