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Biochemical Genetics

, Volume 19, Issue 3–4, pp 263–276 | Cite as

Nitrate reductase activity (in vivo and in vitro) of ditelosomic stocks of wheat (Triticum aestivum L.)

  • P. W. Jones
  • W. J. Whittington
  • C. B. Johnson
Article

Abstract

The nitrate reductase activities (NRA) of 31 ditelosomic stocks were compared with that of the control plant [Chinese Spring (CS) euploid], using in vivo and in vitro assay procedures that had been optimized with respect to the euploid. Fourteen stocks exhibited significant differences in in vivo NRA from that of the euploid; the effect of removal of a chromosome arm was always to increase NRA. Eight of these stocks showed similar effects in vitro, although in three, a casein-sensitive factor had to be eliminated before the difference was expressed. Homoeologous group effects were evident among ditelosomics of groups 2, 4, and 7, while for three chromosomes (2D, 7A, and 7B), removal of either arm resulted in a similar increase in NRA in vivo and probably in vitro.

Key words

nitrate reductase Triticum aestivum ditelosomics 

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • P. W. Jones
    • 1
  • W. J. Whittington
    • 2
  • C. B. Johnson
    • 3
  1. 1.Department of BiochemistryUniversity of LiverpoolLiverpoolU.K.
  2. 2.Department of Physiology and Environmental StudiesUniversity of Nottingham, School of AgricultureSutton BoningtonU.K.
  3. 3.Department of BotanyUniversity of ReadingReadingU.K.

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