Summary
Effects of acutely administered drugs acting on dopamine, muscarine or GABA receptors on the substance P content in rat substantia nigra were examined. Systemic injection of apomorphine caused a significant reduction in the nigral substance P content. This effect was found to be partially inhibited by atropine pretreatment. Haloperidol completely abolished the effect of apomorphine, while sulpiride did not. When administered alone, haloperidol, sulpiride or atropine had no effect on the nigral substance P content. Oxotremopine, the muscarine receptor agonist, reduced substance P content in the rostral half of the substantia nigra. Reduction in the nigral substance P content was also induced by treatment with the GABA receptor antagonist picrotoxin. On the other hand, diazepam increased the substance P content.
These results suggest that the striatonigral substance P neurons could be regulated by dopaminergic, cholinergic and GABAergic systems, and that the dopaminergic influence on the substance P neurons may be mediated, at least in part, by the cholinergic system.
Similar content being viewed by others
References
Besson MJ, Kemel ML, Gauchy C, Glowinski J (1982) Bilateral asymmetrical changes in the nigral release of [3H]GABA induced by unilateral application of acetylcholine in the cat caudate nucleus. Brain Res 241:241–248
Davies J, Dray A (1976) Substance P in the substantia nigra. Brain Res 107:623–627
Dray A (1979) The striatum and substantia nigra: a commentary on their relationships. Neuroscience 4:1407–1439
Dray A, Straughan DW (1976) Synaptic mechanisms in the substantia nigra. J Pharm Pharmacol 28:400–405
Duka T, Wüster M, Herz A (1979) Rapid changes in enkephalin levels in rat striatum and hypothalamus induced by diazepam. Naunyn-Schmiedeberg's Arch Pharmacol 309:1–5
Eison AS, Eison MS, Iversen SD (1982) Behavioural effects of a novel substance P analogue following infusion into the ventral tegmental area and substantia nigra of rat brain. Brain Res 238:137–152
Gale K, Guidotti A, Costa E (1977) Dopaminergic sensitive adenylate cyclase: location in substantia nigra. Science 195:503–505
Govoni S, Yang H-YT, Bosio A, Pasinetti G, Costa E (1982) Possible interaction between cholecystokinin and dopamine. In: Costa E, Trabucchi M (eds) Regulatory peptides: from molecular biology to function. Raven Press, New York, pp 437–444
Groves PM (1983) A theory of the functional organization of the neostraatum and the neostriatal control of voluntary movement. Brain Res Rev 5:109–132
Hanson G, Alphs L, Pradhan S, Lovenberg W (1981a) Response of striatonigral substance P systems to a dopamine receptor agonist and antagonist. Neuropharmacology 20:541–548
Hanson G, Alphs L, Wolf W, Levine R, Lovenberg W (1981b) Haloperidol-induced reduction of nigral substance P-like immunoreactivity: a probe for the interactions between dopamine and substance P neuronal systems. J Pharmacol Exp Ther 218:568–574
Hong JS, Yang H-YT, Costa E (1978) Substance P content of substantia nigra after chronic treatment with antischizophrenic drugs. Neuropharmacology 17:83–85
James TA, Starr MS (1979) Effects of substance P injected into the substantia nigra. Br J Pharmacol 65:423–429
Janicki P, Łypco A, Szczudlik A, Libich J (1981) Substance P levels in the rat forebrain after apomorphine and haloperidol treatment. Pol J Pharmacol Pharm 33:499–502
Jessell TM (1978) Substance P release from the rat substantia nigra. Brain Res 151:469–478
Kato T, Okada M, Koshiya K (1983) Analysis of neuropeptides and their physiological roles with monoamines. In: Parvez S, Nagatsu T, Nagatsu I, Parvez H (eds) Methods in biogenic amine research. Elsevier Biomedical Press, Amsterdam (in press)
Kebabian JW, Calne DB (1979) Multiple receptors for dopamine. Nature 227:93–96
Kelly SE, Iversen SD (1979) Substance P infusion into substantia nigra of the rat: behavioural analysis and involvement of striatal dopamine. Eur J Pharmacol 60:171–179
Koshiya K, Kato T (1982) Time-dependent changes in the immunoreactive substance P content in rat substantia nigra after acute treatment with a neuroleptic drug. Biomed Res 3:244–246
König JF, Klippel RA (1963) The rat brain: a stereotaxic atlas of the forebrain and lower parts of the brain stem. Williams and Wilkins, Baltimore
Lakoski JM, Mohrland JS, Gebhart GF (1980) The effect of morphine on the content of serotonin, 5-hydroxyindole acetic acid and substance-P in the nuclei raphe magnus and reticularis gigantocellularis. Life Sci 27:2639–2644
Lavielle S, Tassin J-P, Thierry A-M, Blanc G, Herve D, Barthelemy C, Glowinski J (1978) Blockade by benzodiazepines of the selective high increase in dopamine turnover induced by stress in mesocortical dopaminergic neurons of the rat. Brain Res 168:585–594
Lisoprawski A, Blanc G, Glowinski J (1981) Activation by stress of the habenulo-interpeduncular substance P neurons in the rat. Neurosci Lett 25:47–51
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Michelot R, Leviel V, Giorguieff-Chesselet MF, Chéramy A, Glowinski J (1979) Effects of the unilateral nigral modulation of substance P transmission on the activity of the two nigro-striatal dopaminergic pathways. Life Sci 24:715–724
Pradhan S, Hanson G, Lovenberg W (1981) Inverse relation of substance-P like immunoreactivity in dorsal raphe nucleus to serotonin levels in pons-medulla following administration of cocaine and 5-hydroxytryptophan. Biochem Pharmacol 30:1071–1076
Pérez de la Mora M, Fuxe K (1977) Brain GABA, dopamine and acetylcholine interactions. 1. Studies with oxotremorine. Brain Res 135:107–122
Pettibone DJ, Wurtman RJ (1980) D-Amphetamine reduces striatal substance P concentrations by presynaptic release of dopamine. Brain Res 186:409–419
Prezewlocki R, Gramsch C, Höllt V, Millan MJ, Osborne H, Herz A (1980) The distribution and release of β-endorphin in relation to certain possible functions. In: Marson CA, Traczyk WZ (eds) Neuropeptides and neural transmission, Raven Press, New York, pp 245–256
Spano PF, Trabucchi M, Di Chiara G (1977) Localization of nigral dopamine-sensitive adenylate cyclase on neurons originating from the corpus striatum. Science 196:1343–1345
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koshiya, K., Kato, T. Acute changes in nigral substance P content induced by drugs acting on dopamine, muscarine and GABA receptors. Naunyn-Schmiedeberg's Arch. Pharmacol. 324, 223–227 (1983). https://doi.org/10.1007/BF00503899
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00503899