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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 318, Issue 1, pp 49–55 | Cite as

5-Methoxyindoles in pineal gland of cow, pig, sheep and rat

  • O. Beck
  • G. Jonsson
  • A. Lundman
Article

Summary

The occurrence and concentration of the four 5-methoxyindoles: 5-methoxytryptamine, 5-methoxytryptophol, 5-methoxyindole-3-acetic acid and melatonin in the pineal gland of pig, cow, sheep and rat was investigated. The analytical method involved the use of deuterated analogues as internal standards and capillary column gas chromatography — mass spectrometry. The analyses of pineal glands obtained during the morning hours revealed the presence of 5-methoxyindole-3-acetic acid and melatonin in nmoles/g and 5-methoxytryptophol and 5-methoxytryptamine in pmoles/g amounts in pig, cow and sheep. In Wistar and Sprague-Dawley strain of rat, melatonin was present at a concentration of about 0.5 pmoles/pineal, a level which was elevated more than five times by treatment with a monoamine oxidase inhibitor. 5-methoxytryptamine was found at a concentration of about 0.03 pmoles/pineal, and was elevated by monoamine oxidase inhibition.

Key words

Indole Tryptamine Pineal gland Gas chromatography-mass spectrometry 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • O. Beck
    • 1
  • G. Jonsson
    • 1
  • A. Lundman
    • 1
  1. 1.Departments of Toxicology and HistologyKarolinska InstitutetStockholmSweden

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