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Separation and micro determination of lipids by thin-layer chromatography followed by densitometry

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Summary

A method is presented for the rapid analysis of mixtures of lipids, particularly those from blood serum. The lipids are separated by thin-layer chromatography on silicagel, then charred with 10% aqueous sulphuric acid and the amount of carbon on the slides is measured densitometrically. 5–10 μg of lipid mixture are needed for determination of the relative amounts of free sterol, sterol esters, glycerol esters, free fatty acids and total phospholipids.

Zusammenfassung

Es wird eine Methode beschrieben zur schnellen Analyse von Lipidgemischen, insbesonders von Lipiden aus Blutserum. 5–10 μg eines Gemisches werden mit Hilfe der Dünnschicht-Chromatographie auf Kieselgel in Sterinester, Glycerinester, freie Steroide, unveresterte Fettsäuren und Phospholipide getrennt. Nach Verkohlung durch Erhitzen mit 10%iger Schwefelsäure werden die verschiedenen Typen densitometrisch bestimmt.

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Authors and Affiliations

  1. Gaubius Institute of the Leyden University, The Netherlands

    C. M. van Gent

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  1. C. M. van Gent
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Additional information

The investigation forms part of a study on the chemical aspects of atherosclerosis, carried out under the auspices of the Netherlands Organisation of Health Research TNO.

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van Gent, C.M. Separation and micro determination of lipids by thin-layer chromatography followed by densitometry. Z. Anal. Chem. 236, 344–350 (1968). https://doi.org/10.1007/BF00503288

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  • DOI: https://doi.org/10.1007/BF00503288

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