Summary
Tolerance to catecholamines in the beta-adrenergic and dopaminergic receptor-adenylate cyclase system from the rat prostatic membrane has been studied. Preincubation of the tissue with isoproterenol produced not only a decrease in beta-adrenergic receptors and its sensitive adenylate cyclase activity, but also a reduction in dopaminergic response. Similarly, exposure to dopamine resulted in a decline both of isoproterenol- and dopamine-stimulated adenylate cyclase activity. A beta adrenergic antagonist, propranolol, exclusively blocked desensitization in the beta-adrenergic and dopaminergic adenylate cyclase system caused by isoproterenol, while dopamine-induced refractoriness was prevented by a dopaminergic antagonist, haloperidol. These results suggest that desensitization of the beta-adrenergic and dopaminergic adenylate cyclase system begins on receptor-occupation by its specific agonist.
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This research was supported by Grant No. 377096 from the Ministry of Education, Science and Culture, Japan
The postgraduate fellow from Department of Neuropsychiatry, St. Marianna University School of Medicine
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Shima, S., Itirai, M. & Asakura, M. Regulation of the adenylate cyclase system by catecholamines in the rat prostate. Naunyn-Schmiedeberg's Arch. Pharmacol. 312, 19–22 (1980). https://doi.org/10.1007/BF00502568
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DOI: https://doi.org/10.1007/BF00502568