Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 327, Issue 3, pp 228–233 | Cite as

Effects of nerve stimulation on enzyme secretion from the in vitro rat pancreas and 3H-release after preincubation with catecholamines

  • Jaipaul Singh
  • Geoffrey T. Pearson
Article

Summary

In the presence of the cholinergic antagonist atropine, electrical field stimulation (FS) (5–20 Hz) caused a marked, reversible increase in the amylase output from superfused rat pancreatic segments. Adrenaline and noradrenaline evoked dose-dependent increases in amylase output which were similar to those produced by FS. The FS- and catecholamine-evoked amylase secretions were abolished by the β-adrenergic antagonist propranolol. The FS-evoked secretion could be abolished by either the removal of external Ca2+ or the application of tetrodotoxin (TTX, 2×10−6 M). FS also resulted in a reversible increase in the fractional efflux of tritium (3H) from rat pancreatic tissues preincubated with either 3H-noradrenaline or 3H-adrenaline. The effects of FS (5–20 Hz) on 3H efflux were abolished by TTX (2×10−6 M). TTX had no effect on the enhancement of 3H efflux caused by elevation of external potassium concentration (high K+, 75 mM). Removal of superfusate Ca2+ completely abolished both the FS- and high K+-induced increases in 3H efflux. These observations suggest that intrinsic nerve stimulation (i.e. FS) results in the Ca2+-dependent release of sympathetic neurotransmitter, noradrenaline, which has a direct secretory action on the rat pancreas. Furthermore, the findings suggest that adrenaline can be taken up by nervous elements. This raises the possibility that uptake and re-release of circulating adrenaline might contribute to the control of rat pancreatic enzyme secretion by the adrenergic nervous system.

Key words

Nerve stimulation Rat pancreas Amylase secretion 3H-Noradrenaline 3H-Adrenaline 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jaipaul Singh
    • 1
  • Geoffrey T. Pearson
    • 1
  1. 1.MRC Secretory Control Research Group, The Physiological LaboratoryUniversity of LiverpoolLiverpoolUK

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