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Biochemical Genetics

, Volume 17, Issue 7–8, pp 683–692 | Cite as

Expression of galactose-1-P-uridyltransferase in Chinese hamster × human galactosemia somatic cell hybrids

  • Robert S. Sparkes
  • T. Mohandas
  • Maryellen C. Sparkes
  • Jeffery D. Shulkin
Article

Abstract

Lymphocytes from a patient with classic galactosemia (GALT deficiency) were hybridized with a Chinese hamster cell line. Electrophoretic evaluation of GALT in 31 independently derived interspecific hybrid clones failed to demonstrate expression of the human GALT gene even when human chromosome 9 was present. Possible mechanisms for this lack of expression are presented.

Key words

somatic cell hybrids gene mapping galactosemia chromosome 9 

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References

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Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Robert S. Sparkes
    • 1
  • T. Mohandas
    • 2
  • Maryellen C. Sparkes
    • 1
  • Jeffery D. Shulkin
    • 2
  1. 1.Division of Medical Genetics, Departments of Medicine, Pediatrics, and Psychiatry, UCLA Center for the Health SciencesUCLA School of MedicineLos Angeles
  2. 2.Division of Medical Genetics, Department of PediatricsUCLA-Harbor General HospitalTorrance

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