, Volume 82, Issue 1, pp 19–24 | Cite as

β-d-N-Acetylglucosaminidase, a new cytochemical marker of human lymphocyte subpopulations

  • E. Orye
  • Y. Benoit
  • L. Roesbeke
  • M. De Wilde
  • M. Van Yper


β-d-N-acetylglucosaminidase staining characteristics of rosetted or non-rosetted normal and malignant lymphoid cells were compared with those observed after nonspecific esterase and acid phosphatase staining. With the three cytochemical techniques a similar staining pattern was observed in T cells (E-rosettes), their subpopulations Tμ and Tγ, B cells and the non-T, non-B cells, as well as in the T cell populations defined with the monoclonal antibodies OKT3,4 and 8. Tμ cells mostly diplayed a “dot-like” reaction, Tγ and the non-T, non-B cells a “fine to heavy granular” reaction, while most B cells were negative. OKT4 and OKT8 positive lymphocytes showed for the larger part a dot-like staining pattern, however, the frequency of cells with a granular pattern was distinctly higher in the OKT8, than in the OKT4 positive cells.

E(+)mIg(−) and E(−)mIg(−) A.L.L. blasts stained either with a dot-like or granular pattern or failed to react when stained cytochemically for β-d-N-acetylglucosaminidase, nonspecific esterase and acid phosphatase activity. Only in a few instances a discrepancy was observed between the types of staining for esterase and acid phosphatase on one hand and those for β-d-N-acetylglucosaminidase on the other.


Acid Phosphatase Staining Pattern Acid Phosphatase Activity Lymphocyte Subpopulation Nonspecific Esterase 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • E. Orye
    • 1
  • Y. Benoit
    • 1
  • L. Roesbeke
    • 1
  • M. De Wilde
    • 1
  • M. Van Yper
    • 1
  1. 1.Laboratory of Cellular Immunology, Department of PediatricsGhent State UniversityGhentBelgium

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