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Histochemistry

, Volume 82, Issue 1, pp 19–24 | Cite as

β-d-N-Acetylglucosaminidase, a new cytochemical marker of human lymphocyte subpopulations

  • E. Orye
  • Y. Benoit
  • L. Roesbeke
  • M. De Wilde
  • M. Van Yper
Article

Summary

β-d-N-acetylglucosaminidase staining characteristics of rosetted or non-rosetted normal and malignant lymphoid cells were compared with those observed after nonspecific esterase and acid phosphatase staining. With the three cytochemical techniques a similar staining pattern was observed in T cells (E-rosettes), their subpopulations Tμ and Tγ, B cells and the non-T, non-B cells, as well as in the T cell populations defined with the monoclonal antibodies OKT3,4 and 8. Tμ cells mostly diplayed a “dot-like” reaction, Tγ and the non-T, non-B cells a “fine to heavy granular” reaction, while most B cells were negative. OKT4 and OKT8 positive lymphocytes showed for the larger part a dot-like staining pattern, however, the frequency of cells with a granular pattern was distinctly higher in the OKT8, than in the OKT4 positive cells.

E(+)mIg(−) and E(−)mIg(−) A.L.L. blasts stained either with a dot-like or granular pattern or failed to react when stained cytochemically for β-d-N-acetylglucosaminidase, nonspecific esterase and acid phosphatase activity. Only in a few instances a discrepancy was observed between the types of staining for esterase and acid phosphatase on one hand and those for β-d-N-acetylglucosaminidase on the other.

Keywords

Acid Phosphatase Staining Pattern Acid Phosphatase Activity Lymphocyte Subpopulation Nonspecific Esterase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • E. Orye
    • 1
  • Y. Benoit
    • 1
  • L. Roesbeke
    • 1
  • M. De Wilde
    • 1
  • M. Van Yper
    • 1
  1. 1.Laboratory of Cellular Immunology, Department of PediatricsGhent State UniversityGhentBelgium

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