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Phalloidin poisoning of isolated hepatocytes: Lack of enzyme release

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Summary

Enzyme release due to phalloidin was studied with isolated hepatocytes and with perfused livers from the rat. In isolated cells the spontaneous loss of lysosomal, mitochondrial and soluble enzymes (E.C. 3.1.1.31; E.C. 3.1.3.2; E.C. 2.6.1.1.; E.C. 2.6.1.2) was not significantly altered by phalloidin whereas the peptide triggers the release of high amounts of the enzymes from perfused livers. The results are discussed in connexion with the failure of phalloidin to induce swelling and vacuolization in isolated hepatocytes.

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References

  • Baur, H.: Isolierung und Charakterisierung von Leberparenchymzellen der Ratte. Diplomarbeit an der Eberhard-Karl-Universität Tübingen (1971)

  • Dolara, P., Buiatti, E., Geddes, M.: Inulin distribution kinetics in normal and phalloidin-poisoned rat livers. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 146–151 (1972)

    Google Scholar 

  • Fishman, W. H., Springer, B., Brunetti, R.: Application of an improved glucoronidase assay; method to the study of human blood β-glucuronidase. J. biol. Chem. 173, 449–456 (1948)

    Google Scholar 

  • Frimmer, M.: The influence of physical conditions on swelling and K+-release in perfused rat livers poisoned with phalloidin. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 393–403 (1972)

    Google Scholar 

  • Frimmer, M., Gries, J., Hegner, D., Schnorr, B.: Studies of the mechanism of the action of phalloidin. Release of lysosomal enzymes and potassium. Naunyn-Schmiedeberg's Arch. Pharmak. exp. Path. 258, 197–214 (1967)

    Google Scholar 

  • Frimmer, M., Herrmann, S., Lutz, F.: Release from the phalloidin poisoned rat liver of a cytotoxic principle producing liver swelling, potassium release and hemolysis. Naunyn-Schmiedeberg's Arch. Pharmacol. 276, 187–197 (1973)

    Google Scholar 

  • Frimmer, M., Kroker, R.: The role of extracellular K+-concentration in phalloidin effects on isolated hepatocytes and perfused rat livers. Naunyn-Schmiedeberg's Arch. Pharmacol. (in press)

  • Gianetto, R. R., De Duve, C.: Tissue fraction studies. 4. Comparative study of the binding of acid phosphatase, β-glucuronidase and cathepsin by rat liver particles. Biochem. J. 59, 433–438 (1955)

    Google Scholar 

  • Jahn, W.: Aufnahme von Wasser und hochmolekularen Substanzen durch die isoliert perfundierte Leber nach Phalloidinvergiftung und nach Erhöhung des posthepatischen Druckes. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 405–418 (1972)

    Google Scholar 

  • Karmen, A., Wroblewski, F., La Due, J. S.: In transaminase activity in human blood. J. clin. Invest. 34, 126–133 (1955)

    Google Scholar 

  • Kroker, R., Hegner, D.: Solubilization of phalloidin binding sites from rat liver plasma membranes by trypsin. Naunyn-Schmiedeberg's Arch. Pharmacol. (submitted for publication)

  • Lutz, F., Glossmann, H., Frimmer, M.: Binding of 3H-Desmethylphalloin to isolated plasma membranes from rat liver. Naunyn-Schmiedeberg's Arch. Pharmacol. 273, 341–351 (1972)

    Google Scholar 

  • Matschinsky, F., Meyer, U., Wieland, O.: Die Wirkung des Knollenblätterpilzgiftes Phalloidin auf die isolierte Rattenleber. Biochem. Z. 333, 48–61 (1960)

    Google Scholar 

  • Matschinsky, F., Wieland, O.: Über Serumveränderungen und Störungen der Mitochondrienfunktion bei experimenteller Phalloidinvergiftung. Biochem. Z. 333, 33–47 (1960)

    Google Scholar 

  • Miller, F., Wieland, O.: Elektronenmikroskopische Untersuchungen der Leber von Maus und Ratte bei akuter Phalloidinvergiftung. Virchows Arch. path. Anat. 343, 83–99 (1967)

    Google Scholar 

  • Puchinger, H.: Zum Wirkungsmechanismus von Phalloidin und Antamanid. Inaug.-Diss., Frankfurt a.M. 1968

  • Puchinger, H., Wieland, T.: Suche nach dem Metaboliten bei Vergiftung mit Desmethylphalloidin. Europ. J. Biochem. 11, 1–6 (1969)

    Google Scholar 

  • Weiss, E., Sterz, J., Frimmer, M., Kroker, R.: Electron microscopy of isolated rat hepatocytes before and after treatment with phalloidin. (in preparation)

  • Wieland, O.: Changes in liver metabolism induced by the poisons of amanita phalloides. Clin. Chem. 2, 323–338 (1965)

    Google Scholar 

  • Wroblewski, F., La Due, J. S.: Serum glutamic pyruvic transaminase in cardiac and hepatic disease. Proc. Soc. exp. Biol. (N.Y.) 91, 569–571 (1956)

    Google Scholar 

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie im Fachbereich Veterinärmedizin, der Justus Liebig-Universität Gießen, Germany

    M. Frimmer & R. Kroker

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  1. M. Frimmer
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  2. R. Kroker
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This work was supported by the Deutsche Forschungsgemeinschaft.

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Frimmer, M., Kroker, R. Phalloidin poisoning of isolated hepatocytes: Lack of enzyme release. Naunyn-Schmiedeberg's Arch. Pharmacol. 279, 99–104 (1973). https://doi.org/10.1007/BF00502073

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  • DOI: https://doi.org/10.1007/BF00502073

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