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Histochemistry

, Volume 40, Issue 3, pp 209–214 | Cite as

Immunocytochemical confirmation of the neural crest origin of avian calcitonin-producing cells

  • Julia M. Polak
  • A. G. E. Pearse
  • C. Le Lièvre
  • J. Fontaine
  • N. M. Le Douarin
Article

Summary

Seventy-five chick embryos (6 to 10-somites) received orthotopie and isochronic grafts of quail neural primordium extending from the mesencephalorhombencephalic constriction down to the 6th somite. Six embryos survived to the 19th or 20th day of incubation. The ultimobranchial glands of the grafted chick embryos contained large numbers of quail cells, distinguished by their characteristic nuclear chromatin.

Antisera to human porcine, salmon and dogfish calcitonins were tested against chick and quail ultimobranchial C cells. Only anti-dogfish calcitonin reacted positively. The biologically marked quail C cells in the chick ultimobranchial glands were shown by immunofluorescence to contain calcitonin.

The production of a polypeptide hormone by neuroectodermal cells derived from the neural crest has thus been established and the derivation of avian calcitonin-secreting C cells from the neural crest confirmed.

Keywords

Public Health Polypeptide Crest Calcitonin Neural Crest 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Julia M. Polak
    • 1
    • 2
  • A. G. E. Pearse
    • 1
    • 2
  • C. Le Lièvre
    • 1
    • 2
  • J. Fontaine
    • 1
    • 2
  • N. M. Le Douarin
    • 1
    • 2
  1. 1.Department of HistochemistryRPMS, Hammersmith HospitalLondonUK
  2. 2.Laboratoire d'Embryologie de l'Université de NantesNantesFrance

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