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Applicability of the isolated perfused rat brain for studying central cholinergic mechanisms

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Summary

The ACh content of the isolated perfused rat brain as well as that of the rat brain in vivo was determined by gas chromatography. After a perfusion period of 30 min the endogenous ACh content of the isolated brain was significantly higher than that of the brain in vivo. Physostigmine caused a rise in the ACh concentration of both the isolated brain and the brain in vivo. Oxotremorine produced an increase in the ACh content in the brain in vivo, but not in the isolated brain after appropriate dosage.

The concentration of the drugs in the perfusion medium of the isolated brain was so that distinct EEG changes could be observed but spontaneous electrical activity could be maintained during the whole perfusion period.

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Author notes

  1. J. Krieglstein

    Present address: Institut für Pharmakologie und Toxikologie im Fachbereich Pharmazie und Lebensmittelchemie der Universität, Deutschhausstraße 17a, D-3550, Marburg/Lahn, Federal Republic of Germany

Authors and Affiliations

  1. Pharmakologisches Institut der Universität, Obere Zahlbacher Straße 67, D-6500, Mainz, Germany

    H. Kilbinger & J. Krieglstein

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  1. H. Kilbinger
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  2. J. Krieglstein
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Kilbinger, H., Krieglstein, J. Applicability of the isolated perfused rat brain for studying central cholinergic mechanisms. Naunyn-Schmiedeberg's Arch. Pharmacol. 285, 407–411 (1974). https://doi.org/10.1007/BF00501469

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  • DOI: https://doi.org/10.1007/BF00501469

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