Summary
On addition to dog blood or plasma about 40% of exogenous nor-adrenaline escaped detection by photofluorimetric methods, when noradrenaline was present in concentrations ranging from 10 to 1000 ng/ml. As shown by bioassay the missing noradrenaline was not inactivated, but rather bound to plasma proteins; this was shown by experiments using labelled noradrenaline and precipitation of proteins, or Sephadex gel filtration. Cellulose acetate electrophoresis demonstrated binding by all protein fractions, alpha 1 and 2 globulins showing the greatest avidity for noradrenaline. Drugs known to be highly bound by proteins did not affect the binding capacity for noradrenaline. It is concluded that plasma noradrenaline values found after injection or infusion of noradrenaline may be only about half of the real values.
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Supported by Instituto de Alta Cultura (Research Project PMC-2).
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Branco, D., Torrinha, J.F. & Osswald, W. Binding of exogenous noradrenaline by the proteins of dog plasma. Naunyn-Schmiedeberg's Arch. Pharmacol. 285, 367–373 (1974). https://doi.org/10.1007/BF00501465
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DOI: https://doi.org/10.1007/BF00501465