Abstract
Ovalbumin (OA) sensitized guinea pigs were repeatedly challenged with 1% OA in saline nebulized ultrasonically at the 0, 10, 20, 60 and 70th min. The intensity of bronchial obstruction was measured by body plethysmography. The first three challenges (0, 10, 20 min) caused strong asthmatic reactions in all animals, the last two (60, 70 min) only mild ones in 10 out of 15 animals. The development of this “tachyphylaxis” was markedly reduced by pretreatment of the animals with cyclooxygenase inhibitors (indomethacin 10 mg/kg intraperitoneally resp. acetylsalicylic acid 10 mg/kg orally 2h before test). The effect of both inhibitors (i.e. inhibition of tachyphylaxis) was abolished by supplementing prostaglandin E2 as aerosol simultaneously to the allergen (100–200 ng per inhalation). The results suggest that allergen tachyphylaxis we have observed in vivo might be due to synthesis of cyclooxygenase products, e.g. prostaglandin E.
Similar content being viewed by others
References
Anderson WH, Krzanowski JJ, Polson JB, Szentivanyi A (1979a) Increased synthesis of prostaglandin-like material during histamine tachyphylaxis in canine tracheal smooth muscle. Biochem Pharmacol 28:2223–2226
Anderson WH, Krzanowski JJ, Polson JB, Szentivanyi A (1979b) Characteristics of histamine tachyphylaxis in canine tracheal smooth muscle. Naunyn-Schmiedeberg's Arch Pharmacol 308:117–125
Austen KF, Orange RP (1975) Bronchial asthma: The possible role of chemical mediators in the pathogenesis of subacute chronic disease. Am Rev Resp Dis 112:423–436
Bito LZ, Baroody RA, Reitz ME (1977) Dependence of pulmonary prostaglandin metabolism on carrier mediated transport processes. Am J Physiol 232:E382-E387
Boot JR, Cockerill AF, Dawson W, Mallen DNB, Osborne DJ (1978) Modification of prostaglandin and thromboxane release by immunological sensitization and succesive immunological challenges from guinea pig lung. Int Archs Allergy Appl Immunol 57:159–164
Boot JR, Dawson W, Cockerill AF, Mallen DNB, Osborne DJ (1977) The pharmacology of prostaglandin-like substances released from guinea pig lung during anaphylaxis. Prostaglandins 11:933–942
Burka JF, Paterson NAM (1980) Enhancement of antigen-induced tracheal contraction by cyclooxygenase inhibition. Adv Prostaglandin Thromboxane Res 8:1755–1758
Dorsch W, Waldherr U, Rosmanith J (1981) Continuous recording of intrapulmonary “compressed air” as a sensitive noninvasive method of measuring bronchial obstruction in guinea pigs. Pflügers Arch 391:236–241
Dunlop LS, Smith AP (1975) Reduction of antigen-induced contraction of sensitized human bronchus in vitro by indomethacin. Br J Pharmacol 54:493–495
Engineer DM, Niederhauser U, Piper PJ, Sirois P (1978) Release of mediators of anaphylaxis: Inhibition of prostaglandin synthesis and the modefication of release of slow reacting substances of anaphylaxis and histamine. Br J Pharmacol 62:61–66
Fingl E, Woodbury DM (1975) Introduction/general principles. In: Goodman LS, Gilman A (eds) The pharmacologic basis of therapeutics. MacMillan Publishing Co Inc., New York, p 27
Goetzl EJ (1980) Occasional notes: Mediators of immediate hypersensitivity derived from arachidonic acid. N Engl J Med 303:822–824
Grodzinska L, Panczenko B, Gryglewski RJ (1975) Generation of prostaglandin E-like material by the guinea pig trachea contracted by histamine. J Pharmacol 27:88–91
Hirata F, Axelrod J (1980) Phospholipid methylation and biological signal transmission. Science 209:1082–1090
Human RL, Spannhake EW, Kadowitz PJ (1978) State of the art: Prostaglandins and the lung. Am Rev Resp Dis 117:111–136
Jesse RL, Franson RC (1979) Modulation of purified phospholipase A2 activity from human platelets by calcium and indomethacin. Biochem Biophys Acta 575:467–470
Kaplan L, Weiss J, Elsbach P (1978) Low concentrations of indomethacin inhibit phospholipase A2 of rabbit polymorphonuclear leukocytes. Proc Natl Acad Sci USA 75:2955–2958
Kennerly DA, Sullivan TJ, Parker CW (1979) Activation of phospholipid metabolism during mediator release from stimulated rat mast cells. J Immunol 122:152–159
Lewis RA, Holgate ST, Roberts LJ, Maguire JF, Oates JA, Austen KF (1979) Effects of indomethacin on cyclic nucleotide levels and histamine release from rat serosal mast cells. J Immunol 123:1663–1668
Mathé AA (1976) Studies on actions of prostaglandins in the lung. Acta Physiol Scand Suppl 441:1–55
Mathé AA, Hedqvist P, Holmgren A, Svanborg N (1973) Bronchial hyperreactivity to prostaglandin F2 and histamine in patients with asthma. Br Med J 1:193–196
Mathé AA, Hedqvist P, Strandberg K, Leslie CA (1977a) Aspects of prostaglandin fuction in the lung. N Engl J Med 296:850–855 (first part), 910–914 (second part)
Mathé AA, Yen SS, Sohn R, Hedqvist P (1977b) Release of prostaglandins and histamine from sensitized and anaphylactic guinea pig lungs — changes in cyclic AMP levels. Biochem Pharmacol 26:181–188
Moncada S, Vane JR (1979) Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2 and prostacyclin. Pharmacol Rev 30:293–331
Needleman Ph (1978) Experimental criteria for evaluating prostaglandin biosynthesis and intrinsic function. Biochem Pharmacol 27:1515–1518
Okazaki T, Ilea VS, Rosario NA, Reisman RE, Arbesman CE, Lee J, Middleton E (1977) Regulatory role of prostaglandin E in allergic histamine release. J Allergy Clin Immunol 60:360–366
Okazaki T, Johnson TF, Reisman RE, Arbesman CE, Middleton E (1978) Plasma prostaglandin concentrations in allergic bronchial asthma. Int Archs Allergy Appl Immunol 57:279–281
Ono T, Motoyama Y, Sakai S, Yoneda J, Kumada S (1979) Interaction between aspirin and prostaglandins in the isolated guinea pig tracheal muscle. Jpn J Pharmacol 29:865–875
Orehek J, Douglas JS, Bouhuys A (1975) contractile responses of the guinea pig trachea in vitro: modification by prostaglandin synthesis inhibiting drugs. J Pharmacol Exp Ther 194:554–564
Piper PJ, Walker JL (1973) The release of spasmogenic substances from human chopped lung and its inhibition. Br J Pharmacol 47:291–304
Platshon LF, Kaliner M (1978) The effect of immunologic release of histamine upon human lung cyclic nucleotide levels and prostaglandin generation. J Clin Invest 62:113–121
Schlumberger HD (1980) Drug induced pseudoallergic syndrome as examplified by acetylsalicylic acid intolerance. In: Dukor et al. (eds) PAR — Pseudoallergic reactions, vol I. Karger, Basel, pp 125–203
Sohn RJ, Mathé AA, Leslie CA (1977) Guinea pig anaphylaxis: time course of changes in cyclic AMP and cyclic GMP. Life Sci 21:1365–1372
Strandberg K, Mathé AA, Yen SS (1977) Release of histamine and formation of prostaglandins in human lung tissue and rat mast cells. Int Archs Allergy Appl Immunol 53:520–523
Stoner J, Manganiello VC, Vaughn M (1973) Effects of bradykinin and indomethacin on cyclic GMP and cyclic AMP in lung slices. Proc Natl Acad Sci USA 70:3830–3833
Sullivan TJ, Parker KL, Eisen SA, Parker ChW (1975) Modulation of cyclic AMP in purified rat mast cells. II. Studies on the relationship between intracellular cyclic AMP-concentrations and histamine release. J Immunol 114:1480–1485
Yen SS, Mathé AA, Dugan JJ (1976) Release of prostaglandins from healthy and sensitized guinea pig lung and trachea by histamine. Prostaglandins 11:227–239
Wasserman MA, Griffin RL, Marsalisi FB (1980) Inhibition of bronchoconstriction by aerosols of prostaglandins E1 and E2. J Pharmacol Exp Ther 214:68–73
Weissmann G, Smolen JE, Korchak H (1980) Prostaglandins and inflammation: Receptor/cyclase coupling as an explanation of why PgEs and PgI2 inhibit functions of inflammatory cells. Adv Prostaglandin Thromboxane Res 8:1637–1653
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dorsch, W., Frey, L. Allergen tachyphylaxis of guinea pigs in vivo: A prostaglandin E mediated phenomenon?. Naunyn-Schmiedeberg's Arch. Pharmacol. 317, 351–356 (1981). https://doi.org/10.1007/BF00501318
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00501318