Taurocholate excretion and bile formation in the isolated perfused rat liver
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The excretory transport maximum (Tm) for taurocholate was determined in the perfused rat liver and compared to that obtained in the intact rat. An in situ liver perfusion system employing a semisynthetic perfusion medium containing Krebs-Ringer-bicarbonate solution, bovine erythrocytes and bovine albumin was used.
In contrast to the bromosulfophthalein (BSP)-Tm reported previously, the taurocholate-Tm was 45% smaller in vitro (196±17 nmol/min per g liver) than in vivo (357±10 nmol/min per g liver), indicating that bile salt transport is more susceptible to alterations induced by the conditions of the perfusion than BSP transport. These findings add to the differences observed previously between the hepatic handling of anionic dyes and bile salts.
At a low taurocholate infusion rat (57 nmol/min per g liver) the normal relationship between bile salt excretion and bile flow observed in vivo was maintained in the perfused liver. At higher taurocholate infusion rates, however, bile flow, for a given bile salt excretion rate, was smaller than in vivo.
These findings should be taken into account when the isolated perfused rat liver is employed for studies of bile formation.
Key wordsLiver Perfusion Bile Salts Biliary Excretion Bile Flow
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