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Taurocholate excretion and bile formation in the isolated perfused rat liver

An in vitro-in vivo comparison
  • G. Paumgartner
  • R. Herz
  • K. Sauter
  • H. P. Schwarz
Article

Summary

The excretory transport maximum (Tm) for taurocholate was determined in the perfused rat liver and compared to that obtained in the intact rat. An in situ liver perfusion system employing a semisynthetic perfusion medium containing Krebs-Ringer-bicarbonate solution, bovine erythrocytes and bovine albumin was used.

In contrast to the bromosulfophthalein (BSP)-Tm reported previously, the taurocholate-Tm was 45% smaller in vitro (196±17 nmol/min per g liver) than in vivo (357±10 nmol/min per g liver), indicating that bile salt transport is more susceptible to alterations induced by the conditions of the perfusion than BSP transport. These findings add to the differences observed previously between the hepatic handling of anionic dyes and bile salts.

At a low taurocholate infusion rat (57 nmol/min per g liver) the normal relationship between bile salt excretion and bile flow observed in vivo was maintained in the perfused liver. At higher taurocholate infusion rates, however, bile flow, for a given bile salt excretion rate, was smaller than in vivo.

These findings should be taken into account when the isolated perfused rat liver is employed for studies of bile formation.

Key words

Liver Perfusion Bile Salts Biliary Excretion Bile Flow 

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References

  1. Alpert, S., Mosher, M., Shanske, A., Arias, I. M.: Multiplicity of hepatic excretory mechanisms for organic anions. J. gen. Physiol. 53, 238–247 (1969)Google Scholar
  2. Dhumeaux, D., Erlinger, S., Benhamou, J. P., Fauvert, R.: Effects of rose bengal on bile secretion in the rabbit: inhibition of a bile salt-independent fraction. Gut 11, 134–140 (1970)Google Scholar
  3. Glasinovic, J. C., Dumont, M., Duval, M., Erlinger, S.: Hepatocellular uptake of taurocholate in the dog. Digestion 8, 432–433 (1973)Google Scholar
  4. Goresky, C. A.: Initial distribution and rate of uptake of sulfobromophthalein in the liver. Amer. J. Physiol. 207, 13–26 (1964)Google Scholar
  5. Goresky, C. A.: The hepatic uptake and excretion of sulfobromophthalein and bilirubin. Canad. med. Ass. J. 92, 851–857 (1965)Google Scholar
  6. Harkavy, M., Javitt, N. B.: Effect of ethinyl estradiol on hepatic excretory function of the rat. In: Metabolic effects of gonadal hormones and contraceptive steroids, pp. 11–18. New York: Plenum Press 1969Google Scholar
  7. Herz, R., Cueni, B., Bircher, J., Paumgartner, G.: The excretory capacity of the isolated rat liver. An in vitro—in vivo comparison. Naunyn-Schmiedeberg's Arch. Pharmacol. 277, 297–304 (1973)Google Scholar
  8. Horak, W., Grabner, G., Paumgartner, G.: Inhibition of bile salt-independent bile formation by indocyanine green. Gastroenterology 64, 1005–1012 (1973)Google Scholar
  9. King, J. E., Schoenfield, L. J.: Cholestasis induced by taurolithocholate in isolated hamster liver. J. clin. Invest. 50, 2305–2312 (1971)Google Scholar
  10. Levi, A. J., Gatmaitan, Z., Arias, I. M.: Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions. J. clin. Invest. 48, 2156–2167 (1969)Google Scholar
  11. Paumgartner, G., Horak, W., Probst, P., Grabner, G.: Effect of phenobarbital on bile flow and bile salt excretion in the rat. Naunyn-Schmiedebergs Arch. Pharmak. 270, 98–101 (1971)Google Scholar
  12. Reichen, J., Paumgartner, G.: Kinetics of bile salt transport by the perfused rat liver. Experientia (Basel) 30, 682–683 (1974)Google Scholar
  13. Reyes, J., Levi, A. J., Gatmaitan, Z., Arias, I. M.: Studies of Y and Z, two hepatic cytoplasmic organic anion-binding proteins: effect of drugs, chemicals, hormones and cholestasis. J. clin. Invest. 50, 2242–2252 (1971)Google Scholar
  14. Schwarz, H. P., Herz, R., Sauter, K., Paumgartner, G.: Taurocholate induced anticholeresis in the rat. Europ. J. clin. Invest. 3, 268 (1973)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • G. Paumgartner
    • 1
  • R. Herz
    • 1
  • K. Sauter
    • 1
  • H. P. Schwarz
    • 1
  1. 1.Institut für Klinische Pharmakologie der Universität BernBernSwitzerland

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